Summary
The fine structure of the parathyroid glands of the anuran, Xenopus laevis Daudin is described during the metamorphosis of larvae and in young adults. Two distinct forms of epithelial cells are found viz.: “dark” and “light” cells and the significance of these cell conditions is considered and discussed. Parathyroid glands from young untreated toads are compared and contrasted with glands from toads maintained for prolonged periods in high concentrations, up to 1%, of calcium chloride in aqueous solution. The development of unusual membranous inclusions in the cytoplasm of the experimentally-treated toads is described.
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References
Bargmann, W.: Die Epithelkörperchen. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. VI/2. Berlin: Springer 1939.
Barrington, E. J. W.: In: An introduction to general and comparative endocrinology. Oxford: Clarendon Press 1963.
Boschwitz, D.: The parathyroids of Bufo viridis Laurenti. Herpetologica 17, 192–199 (1961).
Brehm, H. von: Morphologische Untersuchungen an Epithelkörperchen (Glandulae parathyreoideae) von Anuren. I. Z. Zellforsch. 61, 376–400 (1963).
—: Experimentelle Studie zur Frage jahreszyklischer Veränderungen. Morphologische Untersuchungen an Epithelkörperchen (Glandulae parathyreoideae) von Anuren. II. Z. Zellforsch. 61, 725–741 (1964).
Bruce, H. M., and A. S. Parkes: Rickets and osteoporosis in Xenopus laevis. J. Endocr. 7, 64–81 (1950).
Coleman, R., P. J. Evennett, and J. M. Dodd: Ultrastructural observations on some membranous cytoplasmic inclusion bodies in follicular cells of experimentally-induced goitres in tadpoles and toads of Xenopus laevis Daudin. Z. Zellforsch. 84, 497–505 (1968).
Cortelyou, J. R., and D. J. McWhinnie: Parathyroid glands of amphibians. I. Parathyroid structure and function in the amphibian with emphasis on regulation of mineral ions in body fluids. Amer. Zoologist 7, 843–855 (1967).
Fawcett, D. W.: In: The cell, an atlas of fine structure, p. 293–306. Philadelphia: W. B. Saunders & Co. 1966.
Guardabassi, A.: Les sels de Ca du sac endolymphatique et les processus de calcification des os pendant la métamorphose normale et expérimentale chez les têtards de Bufo vulgaris, Rana dalmatina, Rana esculenta. Arch. Anat. micr. Morph. exp. 42, 143–167 (1953).
—: I sali di calcio del sacco endolinfatico e i processi di ossificazione in girini di Xenopus laevis detimizzati. Arch. ital. Anat. Embriol. 41, 278–296 (1956).
Hara, J., H. Isono, and A. Fujii: Electron microscopic observation of the parathyroid gland of Bufo vulgaris japonicus. [In Japanese; English summary.] Acta Sch. med. Gifu 7, 1548–1556 (1959).
—, and K. Yamada: Chemocytological observations on the parathyroid gland of the toad (Bufo vulgaris japonicus) in specimens taken throughout the year. Z. Zellforsch. 65, 814–828 (1965).
Irving, J. T., and C. M. Solms: The influence of parathyroid hormone upon bone formation in Xenopus laevis. S. Afr. J. med. Sci. 20, 32 (1955).
Isono, H.: Histological study of the parathyroid gland in the toad (Bufo vulgaris japonicus). [In Japanese; English summary.] Acta Sch. med. Gifu 8, 277–293 (1960).
—, S. Isono, and M. Komura: On the seasonal cyclic changes of the glycogen contents in the parathyroid gland of the toad (Bufo vulgaris japonicus). [In Japanese; English summary.] Acta Sch. med. Gifu 7, 1696–1704 (1959).
Kreiner, J.: Saccus endolymphaticus in Xenopus laevis. [In Polish; English summary.] Folia biol. (Kraków) 2, 271–286 (1954).
Lange, R., and H. von Brehm: On the fine structure of the parathyroid gland in the toad and the frog. In: The parathyroid glands, ultrastructure, secretion and function (eds. P. J. Gaillard, R. V. Talmage and A. M. Budy), p. 19–26. Chicago: Univ. Chicago Press 1965.
Matty, A. J.: Endocrine glands in lower vertebrates. Int. Rev. Gen. exp. Zool. 2, 43–138 (1966).
McWhinnie, D. J., and J. R. Cortelyou: Parathyroid glands of amphibians. II. Structural and biochemical changes in amphibian tissues elicited by parathyroid hormone under varying conditions of season and temperature. Amer. Zoologist 7, 857–868 (1967).
Montskó, T., T. Benedeczky, and A. Tigyi: Ultrastructure of the parathyroid gland in Rana esculenta. Acta biol. Acad. Sci. hung. 13, 379–388 (1963a).
—, A. Tigyi, I. Benedeczky, and K. Lissák: Electron microscopy of parathyroid secretion in Rana esculenta. Acta biol. Acad. Sci. hung. 14, 81–94 (1963b).
Nieuwkoop, P. D., and J. Faber: Normal table of Xenopus laevis (Daudin). Amsterdam: North Holland Publ. Co. 1956.
Perrin, A. B., H. I. Bader, A. Tashjian, and P. Goldhaber: Immunofluorescent localization of parathyroid hormone in extracellular spaces of the bovine parathyroid gland. Proc. Soc. exp. Biol. (N.Y.) 128, 219–221 (1968).
Pilkington, J. B., and K. Simkiss: Mobilization of the calcium carbonate deposits in the endolymphatic sacs of metamorphosing frogs. J. exp. Biol. 45, 329–341 (1966).
Robertson, D. R.: The ultimobranchial body in Rana pipiens. VI. Hypercalcemia and secretory activity. Evidence for the origin of calcitonin. Z. Zellforsch. 85, 453–465 (1968).
Rogers, D. C.: An electron microscope study of the parathyroid gland of the frog (Rana clamitans). J. Ultrastruct. Res. 13, 478–499 (1965).
Romeis, B.: Morphologische und experimentelle Studien über die Epithelkörper der Amphibien. I. Die Morphologie der Epithelkörper der Anuren. Z. Anat. Entwickl.-Gesch. 80, 547–578 (1926).
Saxen, L., and S. Toivonen: The development of the ultimobranchial body in Xenopus laevis Daudin and its relation to the thyroid gland and epithelial bodies. J. Embryol. exp. Morph. 3, 376–384 (1955).
Schlumberger, H. G., and D. H. Burk: Comparative study of the reaction to injury. II. Hypervitaminosis D in the frog with special reference to the lime sacs. Arch. Path. 56, 103–124 (1953).
Schrire, V.: Changes in plasma inorganic phosphate associated with endocrine activity in Xenopus laevis. IV. Injection of parathyroid extract into normal and hypophysectomized animals. S. Afr. J. med. Sci. 6, 1–5 (1941).
Seljelid, R.: Endocytosis in thyroid follicle cells. V. On the redistribution of cytosomes following stimulation with thyrotropic hormone. J. Ultrastruct. Res. 18, 479–488 (1967).
Shapiro, B. G.: The topography and histology of the parathyroid glandules in Xenopus laevis. J. Anat. (Lond.) 68, 39–44 (1933).
Sterba, G.: Über die morphologischen und histogenetischen Thymusprobleme bei Xenopus laevis Daudin nebst einigen Bemerkungen über die Morphologie der Kaulquappen. Abhandl. Sächs. Ges. Akad. Wiss., math.-nat. Kl. 44, 1–54 (1950).
Tigyi, A., T. Montskó, L. Komáromy, and K. Lissak: Comparative ultrastructural analysis of the mechanism of secretion. Acta physiol. Acad. Sci. hung. 33, 127–140 (1968).
Waggener, R. A.: A histological study of the parathyroids in the Anura. J. Morphol. Physiol. 48, 1–44 (1929).
—: An experimental study of the parathyroids in the Anura. J. exp. Zool. 57, 13–55 (1930).
Yamada, K.: 2,2′-dihydroxy-6,6′-dinaphthyl disulfide (DDD) diazo blue B reactive granules in the parathyroid gland of the rat and toad. Experientia (Basel) 19, 486–487 (1963).
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I am grateful to Messrs. R. L. Jones and Z. Podhorodecki for their expert technical assistance. I would also like to thank Professor N. Millott for his help and encouragement during the course of this work.
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Coleman, R. Ultrastructural observations on the parathyroid glands of Xenopus laevis Daudin. Z. Zellforsch. 100, 201–214 (1969). https://doi.org/10.1007/BF00343880
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DOI: https://doi.org/10.1007/BF00343880