Abstract
Spleen cells from an SJL mouse immunized with 70'/3 cells, an established pre-B cell line, were fused with cells of the nonsecretor myeloma line NS.1. One established hybridoma cell line (clone K10.6) continuously secreted antibody that recognized a new antigenic specificity tentatively named Ly-m19. This newly found antigen is detectable on both T and B cells. Cytotoxicity assays reveal that 75 percent of the spleen and lymph-node cells, 35 percent of bone-marrow cells, and 15 percent of thymus cells reacted with antibody of clone K10.6. Strains expressing the specificity Ly-m19.1 are characterized by negative reactions and include the strains AKR, CE/J, RF/J, GR/A, SJL, P/J, BDP/J, and LG/J. All other strains so far tested are Ly-m19.2. This strain distribution pattern distinguishes Ly-m19 from any known murine lymphocyte alloantigen, but it parallels the Lyb-2c haplotype. Linkage test of a set of AKXL recombinant inbred strains revealed close linkage of Ly-m19 and Lyb-2 loci on mouse chromosome 4.
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Abbreviations
- LPS:
-
lipopolysaccharide
- B6:
-
C57BL/6
- Con-A:
-
concanavalin A
- MLC:
-
mixed-lymphocyte culture
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The prefix m (monoclonal) is used following a suggestion by Klein and co-workers (1979).
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Tada, N., Kimura, S., Liu, Y. et al. Ly-m19: The Lyb-2 region of mouse chromosome 4 controls a new surface alloantigen. Immunogenetics 13, 539–546 (1981). https://doi.org/10.1007/BF00343721
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DOI: https://doi.org/10.1007/BF00343721