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UV-induced reversion patterns of constitutive and repressed Salmonella histidine auxotrophs

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Summary

An unlinked regulatory mutation hisT1504, causes an approximate 11-fold derepression of the histidine (his) operon and a linked operator constitutive mutation hisO1242 causes an approximate 15-fold derepression. In this study we demonstrate that hisT1504 provokes a significant increase in the UV-induced reversion frequency of his ochre and frameshift mutations. Analysis of revertants derived from frameshift mutants show that this increment in derepressed strains compared to the repressed strains is due to better growth of suppressed revertants by weak frameshift suppressors. The frequency of revertants suppressed by strong frameshift suppressors appears to be the same in repressed and derepressed strains. In contrast, intragenic revertants appear at two-fold decreased frequency in derepressed strains carrying either of the histidine constitutive mutations, hisT1504 or hisO1242. A possible competition is indicated between frequently transcribing RNA polymerase and error-promoting recombinational repair within the histidine operon.

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References

  • Balbinder, E., Callahan, R. III, McCann, P. P., Cordaro, J. C., Weber, A. R., Smith, A. M., Angelosanto, F.: Regulatory mutants of the tryptophan operon of Salmonella typhimurium. Genetics 66, 31 (1970)

    Google Scholar 

  • Berkowitz, D., Hushon, J. M., Whitfield, H. J., Roth, J. R., Ames, B. N.: Procedure for identifying nonsense mutations. J. Bact. 96, 215 (1968)

    Google Scholar 

  • Brenner, M., Ames, B. N.: The histidine operon and its regulation. In: Metabolic regulation (H. J. Vogel, ed.), vol. 5 of: Metabolic pathways, D. Greenberg, ed. p. 349. New York: Academic Press 1971

    Google Scholar 

  • Bridges, B. A., Dennis, R. E., Munson, R. J.: Differential induction and repair of ultraviolet damage leading to true reversion and external suppressor mutations of an ochre codon in Escherichia coli. Genetics 57, 897 (1967)

    Google Scholar 

  • Chang, G. W., Roth, J. R., Ames, B. N.: Histidine regulation in Salmonella typhimurium. VIII. Mutations of the hisT gene. J. Bact. 108, 410 (1971)

    Google Scholar 

  • Herman, R. K., Dworkin, N. B.: Effect of gene induction on the rate of mutagenesis by ICR-191 in Escherichia coli. J. Bact. 106, 545 (1971)

    Google Scholar 

  • Kondo, S.: Mutagenicity versus radiosensitivity in Escherichia coli. Proc. 12th Int. Congr. Genet. II, 126 (1968)

  • Riddle, D. L., Roth, J. R.: Suppressors of frameshift mutations in Salmonella typhimurium. J. molec. Biol. 54, 131 (1970)

    Google Scholar 

  • Riddle, D. L., Roth, J. R.: Frameshift suppressors. II. Genetic mapping and dominance studies. J. molec. Biol. 66, 483 (1972a)

    Google Scholar 

  • Riddle, D. L., Roth, J. R.: Frameshift suppressors. III. Effects of suppressor mutations on transfer RNA. J. molec. Biol. 66, 495 (1972b)

    Google Scholar 

  • Roth, J. R., Antón, D. N., Hartman, P. E.: Histidine regulatory mutants in Salmonella typhimurium I. Isolation and general properties. J. molec. Biol. 22, 305 (1966)

    Google Scholar 

  • Savic, D. J.: A histidine-constitutive mutation decreases the frequency of recombination within the histidine operon of Salmonella typhimurium. Genetics 71, 461 (1972)

    Google Scholar 

  • Savic, D. J., Kanazir, D. T.: The effect of histidine operator-constitutive mutation on UV-induced mutability within the histidine operon of Salmonella typhimurium Molec. gen. Genet. 118, 45 (1972)

    Google Scholar 

  • Singer, C. E., Smith, G. R., Cortese, R., Ames, B. N.: Mutant tRNAHis ineffective in repression and lacking two pseudouridine modifications. Nature (Lond.) New Biol. 238, 72 (1972)

    Google Scholar 

  • Smith, H. O., Levine, M.: A phage gene controlling integration of the prophage. Virology 31, 207 (1967)

    Google Scholar 

  • Straus, D. S., Hartman, P. E., Hartman, Z.: Actions of mutagens on Salmonella: Molecular mutagenesis. In: Molecular and environmental aspects of mutagenesis: Proc. 6th Ann. Rochester Int. Conf. on Environmental Toxicity, Springfield, Ill.: C. C. Thomas Publ., Inc., in press

  • Vogel, H. J., Bonner, D. M.: Acetylornitherase of Escherichia coli: Partial purification and some properties. J. biol. Chem. 218, 97 (1956)

    Google Scholar 

  • Witkin, E. M.: The role of DNA repair and recombination in mutagenesis. Proc. Int. 12th, Congr. Genet. III, 225 (1969)

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Molecular Biology and Endocrynology 090, Institute for Nuclear Sciences “Boris Kidric”, Vinca, 11001, Beograd, Yugoslavia

    Dragutin J. Savic & Dusan T. Kanazir

  2. Department for Molecular Biology and Biochemistry, Faculty of Sciences, University of Beograd, Beograd, Yugoslavia

    Dragutin J. Savic & Dusan T. Kanazir

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  1. Dragutin J. Savic
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  2. Dusan T. Kanazir
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Communicated by R. Devoret

Requests for reprints should be sent to the “Boris Kidric” Institute.

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Savic, D.J., Kanazir, D.T. UV-induced reversion patterns of constitutive and repressed Salmonella histidine auxotrophs. Molec. gen. Genet. 137, 143–150 (1975). https://doi.org/10.1007/BF00341680

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  • DOI: https://doi.org/10.1007/BF00341680

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