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L'Utrastructure du ganglion cervical supérieur du rat

III. Les effets des rayons X

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Summary

The superior cervical sympathetic ganglion of the rat, maintained in vitro in Krebs' solution, may preserve its ability to transmit nervous impulses for more than 48 hours.

Irradiation with X-rays at a dose of approximately 153,000 rad in 30 minutes shortens electrophysiological function of the ganglion. It renders the preparation more susceptible to changes in the chemical make up of Krebs' solution.

From the morphologic point of view, irradiation produces early and severe lesions of satellite cells. The changes within the neuron occur later and affect the nucleus, the lysosomes, Nissl's substance, and, to a lesser degree, the mitochondria. Cell processes, dendrites and axones show the greatest resistance to irradiation.

The synapses do not show any morphologic changes even at a time when the function of the ganglion shows marked deterioration.

Résumé

Le ganglion cervical supérieur du rat, maintenu in vitro dans une solution de Krebs, peut conserver pendant plus de 48 heures sa capacité de transmettre des excitations nerveuses.

L'irradiation aux rayons X. à la dose d'environ 153000 rad en 30 minutes abrège le fonctionement électrophysiologique de la préparation. Elle rend cette dernière plus sensible à des changements de composition de la solution.

Du point de vue morphologique, l'irradiation provoque des lésions graves et précoces des cellules satellites. Les changements au niveau des neurones sont plus tardifs; ils intéressent le noyau, les lysosomes, la substance de Nissl et, à un degré moindre, les mitochondries. Les prolongements cellulaires, dendrites et axones, sont les structures nerveuses qui résistent le plus longtemps.

Les régions synaptiques ne montrent aucune modification morphologique, même lorsque le fonctionnement des préparations est profondément altéré à la suite de l'irradiation.

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Authors and Affiliations

  1. Instituts d'Histologie et de Physiologie de l'Université de Genève et Organisation romande de Recherches neurophysiologiques, Suisse

    W. G. Forssmann, H. Tinguely, J. M. Posternak & Ch. Rouiller

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  1. W. G. Forssmann
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  2. H. Tinguely
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  3. J. M. Posternak
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  4. Ch. Rouiller
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Travail réalisé avec l'aide du Fonds National Suisse pour la Recherche Scientifique.

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Forssmann, W.G., Tinguely, H., Posternak, J.M. et al. L'Utrastructure du ganglion cervical supérieur du rat. Zeitschrift für Zellforschung 72, 325–343 (1966). https://doi.org/10.1007/BF00341539

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