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Agrobacterium tumefaciens TR-DNA encodes a pathway for agropine biosynthesis

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Summary

A pathway for biosynthesis of the crown-gall opine, agropine is proposed and three potential new precursors characterised. The location of genes involved in the three steps of this pathway was determined by site directed insertions and deletions in the TR region of the octopine Ti plasmid, pTiB6Trac. The proposed biosynthetic pathway for agropine which involves three T-DNA genes is in contrast to the biosynthesis of octopine and nopaline where single T-DNA genes are involved.

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References

  • Anet EFLJ (1957) Chemistry of non-enzymic browing. II. Some crystalline amino acid-deoxy-sugars. Aust J Chem 10: 193–197

    Google Scholar 

  • Anet EFLJ, Reynolds TM (1957) Chemistry of non-enzymic browning I Reactions between amino acids, organic acids and sugars in freeze dried apricots and peaches. Aust J Chem 10: 182–192

    Google Scholar 

  • Bagdasarian M, Lury R, Ruckert B, Franklin F, Badasanian MM, Frey J, Timmis KN (1981) Specific purpose plasmid cloning vectors. II. Board host range, high copy number, RSF1010-derived vectors and a host vector system for cloning in Pseudomonas. Gene 16: 237–247

    Google Scholar 

  • Bevan M, Barnes WM, Chilton M-D (1983) Structure and transcription of the nopaline synthase gene region of T-DNA. Nucl Acid Res 11: 369–384

    Google Scholar 

  • Chilton M-D, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempé J (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295: 432–434

    Google Scholar 

  • DeBeuckeleer M, Lemmers M, DeVos G, Willmitzer L, Van Montagu M, Schell J (1981) Further insight on the transferred DNA of octopine crown gall. Mol Gen Genet 183: 282–288

    Google Scholar 

  • DeGreve H, Dhaese P, Seurinck J, Lemmers M, Van Montagu M, Schell J (1982) Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene. J Mol Appl Genet 1: 499–512

    Google Scholar 

  • Depicker A, Stachel S, Dhaese P, Zambryski P, Goodman HM (1982) Nopaline synthase: transcript mapping and DNA sequence. J Mol Appl Genet 1: 561–573

    Google Scholar 

  • deVos G, DeBeuckeleer M, Van Montagu M, Schell J (1981) Restriction endonuclease mapping of the octopine tumour inducing plasmid pTiAch5 of Agrobacterium tumefacians. Plasmid 6: 249–253

    Google Scholar 

  • Dhaese P, DeGreve H, Decraemer H, Schell J, Van Montagu M (1979) Rapid mapping of transposon insertion and deletion mutations in the large Ti plasmids of Agrobacterium tumefaciens. Nucl Acids Res 7: 1837–1849

    Google Scholar 

  • Firmin JL, Fenwick GR (1978) Agropine: A major new plasmid determined metabolite in crown gall tumours. Nature 276: 842–844

    Google Scholar 

  • Guyon P, Chilton M-D, Petit A, Tempé, J (1980) Agropine in “null type” tumours: evidence for generality of the opine concept. Proc Natl Acad Sci USA 77: 2693

    Google Scholar 

  • Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E (1981) Genetic anlysis of crown gall: fine structure analysis of the T-DNA by site directed mutagenesis. Cell 27: 143–153

    Google Scholar 

  • Kerr A, Roberts WP (1976) Agrobacterium: correlations between and transfer of pathogenicity, octopine and nopaline metabolism and bacterium 84 sensitivity. Physiol Plant Pathol 9: 205–211

    Google Scholar 

  • Leemans J, Shaw C, Deblaere R, DeGreve H, Hernalsteens JP, Maes M, Van Montagu M, Schell J (1981) Site directed mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet 1: 149–164

    Google Scholar 

  • Leemans J, Deblaere R, Willmitzer L, DeGreve H, Hernalsteens JP, Van Montagu M, Schell J (1982) Genetic identification, of functions of TL-DNA transcript in octopine crown galls.

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning — a laboratory manual. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Murai N, Kemp JD (1982) T-DNA of pTi15955 form Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumour. Nucl Acid Res 10: 1659–1689

    Google Scholar 

  • Petit A, Tempé J (1978) Isolation of Agrobacterium Ti plasmid regulatory mutants. Mol Gen Genet 167: 145–155

    Google Scholar 

  • Petit A, David C, Dahl G, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Further extension of the opine coucept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190: 204–214

    Google Scholar 

  • Ruvkun G, Ausubel F (1981) A general method for site-directed mutagenesis in prokaryotes. Nature 289: 85–88

    Google Scholar 

  • Salomon F, Deblaere R, Leemans J, Hernalsteens JP, Van Montagu M, Schell J (1984) Genetic identification of functions of TR-DNA in octopine crown galls. EMBO J 3: 141–146

    Google Scholar 

  • Schroder J, Hillebrandt A, Klipp W, Puhler A (1981) Expression of plant tumour-specific proteins in mini cells of E. coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyl transferase. Nucl Acid Res 9: 5187–5702

    Google Scholar 

  • Tate ME, Ellis JG, Kerr A, Tempé J, Murray KE, Shaw J (1982) Agropine: a revised structure. Carbohydrate Res 104: 105–120

    Google Scholar 

  • Tempé J, Guyon P, Petit A, Ellis JG, Tate ME, Kerr A (1980) Preparation et proprietés de mouveaux substrats cataboliques pour deux types de plasmides oncogénes d' Agrobacterium tumefaciens. CR Acad Sci Paris Ser D 1173–1176

  • Tepfer D, Tempé J (1981) Production d'agropine par des racines formeés sous l'action d' Agrobacterium rhizogenes. CR Acad Sci Paris, Ser III, 292: 153–156

    Google Scholar 

  • Thomashow MF, Nutter R, Montoya A, Gordon MP, Nester E (1980) Integration and organisation of Ti plasmid sequences in crown gall tumours. Cell 19: 729–739

    Google Scholar 

  • Trevelyan W, Proctor D, Harrison J (1950) Detection of sugars on paper chromatograms. Nature 166: 444–445

    Google Scholar 

  • Van Haute E, Joos H, Maes M, Warren G, Van Montagu M, Schell J (1983) Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J 2: 411–417

    Google Scholar 

  • Velten J, Willmitzer L, Leemans J, Ellis J, DeBlaere R, Van Montagu M, Schell J (1983) TR genes involved in agropine production. In: Puhler A (ed) Molecular genetics of the bacteriaplant interaction. Springer-Verlag, Berlin Heidelberg New York, pp 303–312

    Google Scholar 

  • Willmitzer L, Sanchez-Serrano J, Buschfeld E, Schell J (1982) DNA from Agrobacterium, rhizogenes is transferred to and expressed in axenic hairy root plant tissues. Mol Gen Genet 186: 16–22

    Google Scholar 

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Communicated by J. Schell

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Ellis, J.G., Ryder, M.H. & Tate, M.E. Agrobacterium tumefaciens TR-DNA encodes a pathway for agropine biosynthesis. Mol Gen Genet 195, 466–473 (1984). https://doi.org/10.1007/BF00341448

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  • DOI: https://doi.org/10.1007/BF00341448

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