Summary
Two different transcripts are synthesized on the DNA of deletion mutant PII of bacteriophage f1 in E. coli cells infected with this miniphage. Both RNA species appear to be primary transcripts and differ by about 100 nucleotides at their 3'OH end. Mapping of these molecules on the miniphage genome suggests that a two-site terminator is active at the end of the I region of transcription of bacteriophage f1.
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References
Alwine CA, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethylpaper and hybridization with DNA probes. Proc Natl Acad Sci USA 74: 5350–5354
Bailey JM, Davidson N (1976) Methylmercury as reversible denaturing agent for agarose gel electrophoresis. Anal Biochem 70: 75–85
Beck E, Zink B (1981) Nucleotide sequence and genome organization of filamentous bacteriophage f1 and fd. Gene 16: 35–58
Calva E, Burgess RR (1980) Characterization of a rho-dependent termination site within the cro gene of bacteriophage. J Biol Chem 255: 11017–11022
Cashman J, Webster RE (1979) Bacteriophage f1 infection of Escherichia coli. Identification and possible processing of f1-infected mRNAs in vivo. Proc Natl Acad Sci USA 76: 1169–1173
Court D, Brady C, Rosenberg M, Wulff DL, Behr M, Mahoney M, Izumi S (1980) Control of transcription termination: a rho dependent termination site in bacteriophage lambda. J Mol Biol 138: 231–254
Denhardt DT (1975) The single-stranded DNA phages. CRC Crit Rev Microbiol 4: 161–223
Dotto GP, Enea V, Zinder ND (1981) Functional analysis of bacteriophage f1 intergenic region. Virology 114: 463–473
Edens L, Konings RNH, Schoenmakers JGG (1975) Physical mapping of the central terminator for transcription on the bacteriophage M13 genome. Nucl Acid Res 2: 1811–1820
Enea V (1976) Gene-less mutants and the mechanism of genetic recombination in bacteriophage f1. Doctoral dissertation, The Rockefeller University, NY, p 15
Enea V, Zinder ND (1975) A deletion mutant of bacteriophage f1 containing no intact cistrons. Virology 68: 105–114
Enea V, Zinder ND (1982) Interference resistant mutants of phage f1. Virology 122: 222–226
Enea V, Horiuchi K, Turgeon BG, Zinder ND (1977) Physical map of defective interfering particles of bacteriophage f1. J Mol Biol 111: 395–414
Horiuchi K, Vovis GF, Model P (1978) The filamentous phage genome: Genes, physical structure and protein products. In: Denhardt DT, Dressler D, Ray DS (eds). The single-stranded DNA phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 113–137
Jacob E, Hofschneider PH (1969) Replication of the single-stranded DNA bacteriophage M13: messenger RNA synthesis directed by replicative form DNA. J Mol Biol 46: 359–363
Kolter R, Yanofsky C (1982) Attenuation in amino acid biosynthetic operons. Annu Rev Genet 16: 113–134
Konings RHN, Schoenmakers JGG (1978) Transcription of the filamentous phage genome. In: Denhardt DT, Dressler D and Ray DS (eds) The Single-stranded DNA phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 507–530
Kupper H, Sekiya T, Rosenberg M, Egan J, Landy A (1978) A rho dependent termination site in the gene coding for tyrosine tRNA su3 of E. coli. Nature 272: 423–428
La Farina M (1983) Transcription in bacteriophage f1-infected Escherichia coli: Very large RNA species are synthesized on the phage DNA. Mole Gen Genet 191: 22–25
La Farina M, Model P (1978) Transcription in bacteriophage f1-infected Escherichia coli. I. Translation of the RNA in vitro. Virology 86: 368–375
La Farina M, Model P (1983) Transcription in bacteriophage f1-infected Escherichia coli. Messenger populations in the infected cells. J Mol Biol 164: 377–393
Marvin DA, Hohn B (1969) Filamentous bacterial visuses. Bacteriol Rev 33: 172–209
Meyer TF, Geider K, Kurz Ch, Schaller H (1979) Cleavage site of bacteriophage fd gene II protein in the origin of viral strand replication. Nature 278: 365–366
Model P, Zinder ND (1974) In vitro synthesis of bacteriophage f1 proteins. J Mol Biol 83: 231–251
Pratt D, Tzagoloff H, Erdhal WS (1966) Conditional lethal mutants of small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action. Virology 30: 397–410
Ravetch JV, Horiuchi K, Zinder ND (1979) DNA sequence analysis of the defective particles of bacteriophage f1. J Mol Biol 128: 265–450
Ray DS (1978) In vivo replication of filamentous phage DNA. In: Denhardt DT, Dressler D and Ray DS (eds) The single-stranded DNA phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 325–339
Rigby PW, Dickmann M, Rhodes C, Berg P (1977) Labeling de-oxyribonucleic acid to high specific activity in vitro by nick translation with DNA Polymerase I. J Mol Biol 113: 237–251
Rivera MJ, Smits MA, Quint W, Schoenmakers JGG, Konings RNH (1978) Expression of bacteriophage M13 DNA in vivo. Localization of the transcription initiation and termination signal of the mRNA coding for the major capsid protein. Nucl Acid Res 5: 2895–2912
Roberts J (1969) Termination factor for RNA synthesis. Nature 224: 1168–1174
Rosenberg M, Court D, Wulff DL, Shimatake H, Brady C (1978) The relation between function and DNA sequence in an intercistronic regulatory region in the phage. Nature 272: 414–423
Schaller H (1978) The intergenic region and the origins for filamentous phage DNA replication. Cold Spring Harbor Symp Quant Biol 43: 401–408
Smits MA, Simons G, Konings RNH, Schoenmakers JGG (1978) Expression of bacteriophage M13 in vivo. I. Synthesis of phage specific RNA and protein in minicells. Biochem Biophys Acta 521: 27–44
Smits MA, Schoenmakers JGG, Konings RNH (1980) Expression of bacteriophage M13 in vivo. Isolation, identification and characterization of phage specific mRNA species. Eur J Biochem 112: 309–321
Sugimoto K, Sugisaki H, Okamoto T, Takanami M (1977) Studies on bacteriophage fd DNA. The sequence of messenger RNA for major coat protein gene. J Mol Biol 62: 81–88
Vovis GF, Horiuchi K, Zinder ND (1975) Endonuclease R·EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promoter for gene VIII. J Virol 16: 674–684
Wu AM, Christie G, Platt T (1981) Tandem termination sites in the tryptophan operon of Escherichia coli. Proc Natl Acad Sci USA 78: 2913–2917
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La Farina, M., Vitale, M. & Enea, V. Transcription in bacteriophage f1-infected Escherichia coli: RNA synthesized on DNA of deletion mutant PII shows the existence of a two-site terminator. Mol Gen Genet 195, 411–417 (1984). https://doi.org/10.1007/BF00341441
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DOI: https://doi.org/10.1007/BF00341441