Abstract
Male Sprague-Dawley rats were fed a diet containing 1.2% butylated hydroxytoluene (BHT) for 1–7 days, and blood coagulation factors II, VII, VIII, IX and X, and platelet aggregation were measured. The plasma concentrations of factors II, VII, IX and X were significantly reduced in a time-dependent fashion when BHT was administered for 2–7 days and haemorrhages in epididymis were found in rats given BHT for 4–7 days. On the contrary, thrombin-induced and calcium-required aggregation of washed platelets was unchanged throughout the experiment. These results suggest that factors II, VII, IX and X rapidly decrease immediately after the administration of BHT, but hypoaggregability of washed platelets reported previously may be a secondary defect caused by bleeding.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Botham CM, Conning DM, Hayes J, Litchfield MH, McElligot TF (1970) Effect of butylated hydroxytoluene on the enzyme activity and ultrastructure of rat hepatocytes. Fd Cosmet Toxicol 8: 1–8
Francis JL, Armstrong DJ (1982) Acquired dysfibrinogenaemia in liver disease. J Clin Pathol 35: 667–672
Gad SC, Weil CS (1982) Statistics for toxicologists. In: Hayes AW (ed) Principles and method of toxicology. Raven Press, New York p 237
Gilbert D, Golberg L (1965) Liver response test. III. Liver enlargement and stimulation of microsome processing enzyme activity. Fd Cosmet Toxicol 3: 417–432
Kawano S, Nakao T, Hiraga K (1980) Species and strain differences in the butylated hydroxytoluene (BHT)-producing induction of hepatic drug oxidation enzymes. Jpn J Pharmacol 30: 861–870
Kerr R, Lefevre A, Lane B, Liever CS (1966) Liver growth induced by butylated hydroxytoluene. Clin Res 14: 299
Lane BP, Lieber CS (1967) Effect of butylated hydroxytoluene on the ultrastructure of rat hepatocytes. Lab Invest 16: 342–348
Nakagawa Y, Tayama K, Nakao T, Hiraga K (1984) On the mechanism of butylated hydroxytoluene-induced hepatic toxicity in rats. Biochem Pharmacol 33: 2669–2674
Owen CA Jr, Bowie EJW (1978) Rat coagulation factors, V, VIII, XI and XII: Vitamin K dependent. Haemostasis 7: 189–201
Takahashi O (1985) Reduced aggregation capacity of washed platelets and dysfibrinogenaemia in the rat given butylated hydroxytoluene. Fd Chem Toxicol (in press)
Takahashi O, Hayashida S, Hiraga K (1980) Species differences in the haemorrhagic response to butylated hydroxytoluene. Fd Cosmet Toxicol 18: 229–235
Takahashi O, Hiraga K (1978a) Dose-response study of haemorrhagic death by dietary butylated hydroxytoluene (BHT) in male rats. Toxicol Appl Pharmacol 43: 399–406
Takahashi O, Hiraga K (1978b) Effects of low levels of butylated hydroxytoluene on the prothrombin index of male rats. Fd Cosmet Toxicol 16: 475–477
Takahashi O, Hiraga K (1978c) The relationship between hemorrhage induced by butylated hydroxytoluene and its antioxidant properties or structural characteristics. Toxicol Appl Pharmacol 46: 811–814
Takahashi O, Hiraga K (1981) Haemorrhagic toxicosis in rats given butylated hydroxytoluene. Acta Pharmacol Toxicol 49: 14–20
Takahashi O, Hiraga K (1982) Effects of butylated hydroxytoluene (BHT) on Fitzgerald factor, Fletcher factor and complement fixation activity of rat plasma. Ann Rep Tokyo Metr Res Lab PH 33: 423–428
Takahashi O, Hiraga K (1984) Effects of dietary butylated hydroxytoluene on functional and biochemical properties of platelets and plasma preceding the occurrence of haemorrhage in rats. Fd Chem Toxicol 22: 97–103
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takahashi, O. Feeding of butylated hydroxytoluene to rats caused a rapid decrease in blood coagulation factors II (prothrombin), VII, IX and X. Arch Toxicol 58, 177–181 (1986). https://doi.org/10.1007/BF00340978
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00340978