Abstract
(Bis[tri-n-butyltin])oxide (TBTO) was studied for its toxic potential on prenatal development. The effect of this substance on limb differentiation (mouse embryos) in organ culture was evaluated.
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(a)
In the organ culture system using mouse limb buds, TBTO interfered with morphogenetic differentiation at doses as low as 0.03 μg/ml (5×10−8M). This is one of the lowest concentrations of a substance ever found to be active in this in vitro system.
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(b)
The in vitro studies suggest a high embryotoxic potential of TBTO. The low embryotoxicity found in the in vivo studies may be due to limited exposure of the embryos by the original unmetabolized substance.
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Krowke, R., Bluth, U. & Neubert, D. In vitro studies on the embryotoxic potential of (bis[tri-n-butyltin])oxide in a limb bud organ culture system. Arch Toxicol 58, 125–129 (1986). https://doi.org/10.1007/BF00340970
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DOI: https://doi.org/10.1007/BF00340970