Summary
By employing Pulsed Field Gel Electrophoresis we have shown that the chromosomal DNA pattern of a petite-positive strain of Kluyveromyces lactis, designated KF4, differs from the karyotype of the reference strain. Digestion with SfiI and hybridization with an rDNA probe, demonstrate that a novel chromosomal band in KF4 results from a deletion of 35–40 rDNA cistrons from the rDNA cluster. The significance of this finding to possible alterations in cell physiology and the ability to generate “petite” mutants is discussed.
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References
Clark-Walker GD (1989) Yeast 5:S247-S254
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Galeotti CL, Williams KL (1978) J Gen Microbiol 104, 337–341
Galeotti CL, Clark-Walker GD (1983) In: Nagley P, Linnane AW, Peacock WJ, Pateman JA (eds) Manipulation and expression of genes in eukaryotes. Academic Press, Sydney, pp 159–166
Hardy CM, Galeotti CL, Clark-Walker GD (1989) Curr Genet 16:419–427
Johnston JR, Contopoulou CR, Mortimer R (1988) Yeast 4:191–198
Lai E, Birren BW, Clark SM, Simon MI, Hood L (1989) Biotechnol 7:34–42
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory, New York
McClelland M, Hanish J, Nelson M, Patel Y (1988) Nucleic Acids Res 16:364
Schwartz DC, Cantor CR (1984) Cell 37:67–75
Sor F, Fukuhara H (1989) Yeast 5:1–10
Stark MJR, Milner JS (1989) Yeast 5:35–50
Verbeet MP, Heerikhuizen van H, Klootwijk J, Fontijn RD, Planta RJ (1984) Mol Gen Genet 195:116–125
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Maleszka, R., Clark-Walker, G.D. A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster. Curr Genet 16, 429–432 (1989). https://doi.org/10.1007/BF00340722
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DOI: https://doi.org/10.1007/BF00340722