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The neurosecretory system of the adult Calliphora erythrocephala

II. Histology of the neurosecretory cells of the brain and some related structures

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Summary

Neurosecretory cells in the brain and suboesophageal ganglion of the adult female Calliphora erythrocephala have been studied in the light microscope. The paraldehydefuchsin stain (PAF) gave by far the clearest pictures.

The medial neurosecretory cells of the protocerebrum (m.n.c.) show definite cyclic changes as to the size of the nuclei and the content of secretory material. The cytological changes depend on the age of the fly and the diet given and are correlated with ovarian development.

The nuclear size is held to express the metabolic activity of the cells. Cells with large nuclei, as found in young sugar-flies (S1D) and meat-fed flies with developing ovaries (S4D/M2D), contain less secretory material which is released through the axons, while the m.n.c. of old sugar-flies (S6D) have small nuclei and are stuffed with secretory material which is stored in the perikarya.

These results confirm those obtained by darkfield microscopy of living m.n.c. by Lea and E.Thomsen (1962 and unpublished).

No really convincing evidence for the existence of more than one type of m.n.c. was found.

Two small groups of “lateral cells” were observed. Possibly neurosecretory are further: 1-(2) cells at the base of each optic lobe, two groups of 2–3 cells on the caudal side of the brain, and 2 cells ventrally in the suboesophageal ganglion.

“Giant neurons” of unknown function are situated very near the m.n.c. Their axons join those from the m.n.c., but end in the suboesophageal ganglion.

The same region comprises a number of peculiar cells, each containing a large, fluid-filled vacuole (“the vacuolated cells”). Similar cells are associated with the possibly neurosecretory cells on the caudal side of the brain.

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  1. Zoological Laboratory, Royal Veterinary and Agricultural College, Copenhagen

    Mathias Thomsen

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  1. Mathias Thomsen
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My sincere thanks are due to my wife Dr. Ellen Thomsen, who made all the excisions of the brains and the measurements of the nuclei, to T.C.Normann for valuable assistance with the photographic work, and to Mrs. K. Bahnert for technical help with the gallocyanin method. The Carlsberg Foundation has supported the work with grants.

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Thomsen, M. The neurosecretory system of the adult Calliphora erythrocephala . Zeitschrift für Zellforschung 67, 693–717 (1965). https://doi.org/10.1007/BF00340332

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