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Irradiation of cells in tissue culture

II. Cinematographic analyses of cell enlargement and mitotic activity following gamma irradiation at 2000 r and 4000 r

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Summary

Strain cultures of the cervical carcinoma HeLa (Puck-clone), human fetal intestinal epithelium (Henle) and adult human skin (NCTC clonal 2414) were used in Rose chambers for gamma irradiation at 2000 r and 4000 r from a Cobalt60 source.

Phase contrast, time-lapse cinematographic records generally made from one to 5 days following irradiation yielded a total of more than 6000 feet of 16 mm film records for analysis.

Cell enlargement was regularly observed. Telo-reduplication, including a second division is reported. Multinucleation arising from cells with single and multiple nuclei producing one or two daughter cells with numerous micronuclei was found for all three strains.

It is believed that these mitotic anomalies represented a quantitative rather than a qualitative difference between irradiated and control cultures.

The method permits an accurate assessment of the divisional potentialities of living cells during long periods of life in vitro under experimental conditions.

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Bibliography

  • Canti, R. G.: Demonstration of a cinematograph film of living tissue and the effects of radium irradiation on the cells. Acta radiol. (Stockh.) Suppl. 32, 193 (1928).

    Google Scholar 

  • Gey, G. O.: Effects of irradiation on tissue cultures. (Cinemicrograph.) East. Conf. Radiol., 1936 (not published).

  • Hsu, T. C., and P. S. Moorhead: Chromosome anomalies in human neoplasms with special reference to the mechanisms of polyploidization and aneuploidization in the HeLa strain. Ann. New York Acad. Sci. 63, 1083–1094 (1956).

    Google Scholar 

  • Leone, V., T. C. Hsu u. C. M. Pomerat: Cytological studies on HeLa, a strain of human cervical carcinoma. II. On rotatory movements of the nuclei. Z. Zellforsch. 41, 481–492 (1955).

    Google Scholar 

  • Levan, A., and T. S. Hauschka: Endomitotic reduplication mechanisms in ascites tumors of the mouse. J. Nat. Canc. Inst. 14, 1–43 (1953a).

    Google Scholar 

  • —: Nuclear fragmentation — A normal feature of the mitotic cycle of lymphosarcoma cells. Hereditas (Lund) 39, 137–148 (1953b).

    Google Scholar 

  • Mazia, D.: The relation of the morphology of the cell to its functional behavior following irradiation, pp. 143 to 178. In: Cellular aspects of basic mechanisms in radiobiology. NAS-NRC Publ. No. 450, (Nuclear Science Series Report No. 18), 1956.

  • Nakanishi, Y. H., and H. Nakahara: Cytological studies of tumors. XVII. A phase microscopy study on the mitotic process in the MTK-IV tumor and the Watanabe ascites hepatoma. J. Fac. Sci., Hokkaido Univ., Ser. VI 12 (in press).

  • Pomerat, C. M.: Living human histology. XVe Congr. Soc. Intl. de Chirurgie, Lisbon, Sept. 1953, pp. 236–256 (1954).

  • Pomerat, C. M., S. P. Kent u. L. C. Logie: Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants. Z. Zellforsch. 47, 158–174 (1957).

    Google Scholar 

  • Pomerat, C. M., C. G. Lefeber and McD. Smith: Quantitative cine analysis of cell organoid activity. Ann. New York Acad. Sci. 58, 1311–1321 (1954).

    Google Scholar 

  • Pomerat, C. M., u. R. R. Overman: Electrolytes and plasma expanders. I. Reaction of human cells in perfusion chambers with phase contrast, time-lapse cine records. Z. Zellforsch. 45, 2–17 (1956).

    Google Scholar 

  • Pomerat, C. M., and McD. Smith: Moving pictures of body cells at work. Research Reviews, ONR, p. 17–21, August 1954.

  • Simon-Reuss, I., and F. G. Spear: The effect of gamma radiation on mitosis in vitro. Brit. J. Radiol. 20, 63–70 (1947).

    Google Scholar 

  • Stroud, A. N.: The effects of continuous irradiation by tritium on cells cultivated in vitro. Ann. New York Acad. Sci. 67, 11–34 (1956).

    Google Scholar 

  • Zirkle, R. E.: Cellular changes following irradiation, pp. 1–45. In: Cellular aspects of basic mechanisms in radiobiology. NAS-NRC Publ. No. 450 (Nuclear Science Series Report No. 18), 1956.

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Authors and Affiliations

  1. Tissue Culture Laboratory, Department of Anatomy, the University of Texas Medical Branch, Galveston, Texas

    C. M. Pomerat, S. P. Kent & L. C. Logie

  2. the Radiobiological Laboratory of the University of Texas and the United States Air Force, Austin, Texas

    C. M. Pomerat, S. P. Kent & L. C. Logie

Authors
  1. C. M. Pomerat
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  2. S. P. Kent
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  3. L. C. Logie
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Additional information

This research was supported by the USAF under Contract No. AF 18(600)-1263, monitored by the School of Aviation Medicine, USAF, Randolph Air Force Base, Texas.

Captain, USAF (MSC).

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Pomerat, C.M., Kent, S.P. & Logie, L.C. Irradiation of cells in tissue culture. Zeitschrift für Zellforschung 47, 175–197 (1957). https://doi.org/10.1007/BF00340147

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  • DOI: https://doi.org/10.1007/BF00340147

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