Summary
Giant cell formation was induced within 12 days in 9 species of human cell strains by exposure to 2000 r and 4000 r of gamma radiation from a Cobalt60 source. In contrast, cells from primary explants derived from 6 different sources and in vitro for less than one month did not exhibit this response.
Cells in the strain cultures have all been shown to be heteroploid, having an average chromosome number ranging from approximately 67 to 83. This factor may serve to explain why they are prone to develop giant cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Bibliography
Auxier, J. A.: A high level Co60 irradiation facility. School of Aviation Medicine, USAF, Report No. 55-40, 1955.
Bassett, C. A. L., V. J. Evans and W. R. Earle: Characteristics and potentials of long term cultures of human skin. Plast. Reconstr. Surg. 17, 421–429 (1956).
Chang, S.: Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exper. Biol. a. Med. 87, 440–443 (1954).
Eagle, H.: Propagation in a fluid medium of a human epidermoid carcinoma, Strain KB. Proc. Soc. Exper. Biol. a. Med. 89, 362–364 (1955).
Frisch, A. W., V. Jentoft, R. Barger and E. J. Losli: A human epithelium-like cell (Maben) derived from an adenocarcinoma of lung. Amer. J. Clin. Path. 25, 1107–1112 (1955).
Hsu, T. C., and P. S. Moorhead: Mammalian chromosomes in vitro VII. Heteroploidy in human cell strains. J. Nat. Canc. Inst. 18, 463–471 (1957).
Hsu, T. C., C. M. Pomerat and P. S. Moorhead: Mammalian chromosomes in vitro. VIII. Heteroploid transformation in the human cell strain Mayes. J.Nat. Canc. Inst. (19, 867 bis 873 (1957).
Koller, P. C.: The effect of radiation on the normal and malignant cells in man. Brit. J. Radiol. Suppl. 1, 84–96 (1947).
Moore, Alice E.: Criteria of Malignancy. II. Biological consideration of normal and abnormal cell lines. Trans. N. Y. Acad. Sci., Ser. II 19, 435–446 (1957).
Paterson, Edith: A comparison of the action of X and gamma radiation on fibroblasts. Brit. J. Radiol. 15, 257–263 (1942).
Perry, V. P., V. J. Evans, W. R. Earle, G. W. Hyatt and W. C. Bedell: Long-term tissue culture of human skin. Amer. J. Hyg. 63, 52–58 (1956).
Perry, V. P., K. K. Sanford, V. J. Evans, G. W. Hyatt and W. R. Earle: Establishment of clones of epithelial cells from human skin. J. Nat. Canc. Inst. 18, 709–718 (1957).
Puck, T. T., and P. I. Marcus: Action of x-rays on mamalian cells. J. of Exper. Med. 103, 653–666 (1956).
Rose, G. G.: A separable and multipurpose tissue culture chamber. Texas Rep. Biol. a. Med. 12, 1074–1083 (1954).
Simon-Reüss, I., and F. G. Spear: The effect of gamma radiation on mitosis in vitro. Brit. J. Radiol. 20, 63–70 (1947).
Spear, F. G.: Tissue culture and experimental radiology. Bristol: John Wright & Sons, Ltd. 1948.
Stroud, A. N., and A. M. Brues: Radiation effects in tissue culture. Texas Rep. Biol. a. Med. 12, 931–944 (1954).
Author information
Authors and Affiliations
Additional information
This research was supported by the USAF under Contract No. AF 18 (600)-1263, monitored by the School of Aviation Medicine, USAF, Randolph Air Force Base, Texas.
Captain, USAF (MSC).
Rights and permissions
About this article
Cite this article
Pomerat, C.M., Kent, S.P. & Logie, L.C. Irradiation of cells in tissue culture. Zeitschrift für Zellforschung 47, 158–174 (1957). https://doi.org/10.1007/BF00340146
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00340146