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Alterations in the fine structure of cytoplasmic organelles in the hepatopancreatic cells of shell-regenerating snail, Helix pomatia L.

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Summary

The investigation of hepatopancreatic cells following damage to the shell revealed hypertrophic alterations of several Cytoplasmic organelles. The network of endoplasmic reticulum was extensively developed within both digestive and calcium cells. The arrangement of tubules exhibited a peculiar hexagonal pattern. It is suggested that the reticulum is of agranular type and may be engaged in the transport of lipids and calcium ions. Remarkable alterations were observed in mitochondria, apparently as the result of their hyperfunction. Mitochondria with reduced cristae, with two-layered transverse septum, and with bleb-like protrusions, occurred frequently. In the calcium cells, helically entwined fibres appeared among the fragments of disintegrated calcium spherites. The results of the investigation and the possible presence of collagen in the fibres originating from disintegrated spherites are discussed.

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References

  • Abolinš-Krogis, A.: The morphological and chemical characteristics of organic crystals in the regenerating shell of Helix pomatia L. Acta Zool. (Stockh.) 39, 19–38 (1958).

    Google Scholar 

  • —: The histochemistiy of the hepatopancreas of Helix pomatia (L.) in relation to the regeneration of the shell. Arkiv Zool. 13, 159–201 (1961).

    Google Scholar 

  • —: On the protein stabilizing substances in the isolated b-granules and in the regenerating membranes of the shell of Helix pomatia (L.). Arkiv Zool. 15, 475–484 (1963a).

    Google Scholar 

  • - The morphological and chemical basis of the initiation of calcification in the regenerating shell of Helix pomatia (L.). Acta Universitatis Upsaliensis No 20, 1–22 (1963b).

  • —: Electron microscope observations on calcium cells in the hepatopancreas of the snail, Helix pomatia, L. Arkiv Zool. 18, 85–92 (1965).

    Google Scholar 

  • —: Shell regeneration in Helix pomatia with special reference to the elementary calcifying particles. Symp. zool. Soc. London No 22, 75–92 (1968).

    Google Scholar 

  • - Electron microscope studies of the intracellular origin and formation of calcifying granules and calcium spherites in the hepatopancreas of the snail, Helix pomatia, L. Z. Zellforsch. 108 (1970).

  • Blanchette, E.J.: Ovarian steroid cells. II. The lutein cell. J. Cell Biol. 81, 517–542 (1966).

    Google Scholar 

  • Burger, J.W., Hess, W.N.: Function of the rectal gland in the spiny dog fish. Science 131, 670–671 (1960).

    Google Scholar 

  • Christensen, A.K., Fawcett, D.W.: The normal fine structure of opossum testicular interstitial cells. J. biophys. biochem. Cytol. 9, 653–670 (1961).

    Google Scholar 

  • —: The fine structure of testicular interstitial cells in guinea pig. J. Cell Biol. 26, 911–935 (1965).

    Google Scholar 

  • Claude, A.: The morphology and significance of dumbbell-shaped mitochondria in early stages of regenerating liver. J. Cell Biol. 27, 146A (Abstract) (1965).

    Google Scholar 

  • Copeland, D.E., Dalton, A.J.: An association between mitochondria and the endoplasmic reticulum in cells of the pseudobranch gland of a teleost. J. biophys. biochem. Cytol. 5, 393–396 (1959).

    Google Scholar 

  • Doyle, W.L.: The principal cells of the salt-gland of marine birds. Exp. Cell Res. 21, 386–393 (1960).

    Google Scholar 

  • Enders, A.C.: Observations on the fine structure of lutein cells. J. Cell Biol. 12, 101–113 (1962).

    Google Scholar 

  • Estabrook, R.W., Cooper, D.Y., Rosenthal, O.: The light reversible carbon monoxide inhibition of the steroid C21-hydroxylase system of the adrenal cortex. Biochem. Z. 388, 741–755 (1963).

    Google Scholar 

  • Fawcett, D.W.: Observations on the cytology and electron microscopy of hepatic cells. J. nat. Cancer Inst., Suppl. 15, 1475–1483 (1955).

    Google Scholar 

  • —, Burgos, M.H.: Studies on the fine structure of the mammalian testis. I. The human interstitial tissue. Amer. J. Anat. 107, 245–269 (1960).

    Google Scholar 

  • Godman, G.C., Porter, K.R.: Chondrogenesis, studied with the electron microscope. J. biophys. biochem. Cytol. 8, 719–760 (1960).

    Google Scholar 

  • Ito, S., Winchester, R.J.: The fine structure of the gastric mucosa in the bat. J. Cell Biol. 16, 541–577 (1963).

    Google Scholar 

  • Jones, A.L., Fawcett, D.W.: Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in liver). J. Histochem. Cytochem. 14, 215–232 (1966).

    Google Scholar 

  • Komnick, H.: Elektronmikroskopische Untersuchungen zur funktionellen Morphologie des Ionentransportes in der Salzdrüse von Larus argentatus. Protoplasma (Wien) 56, 274–314 (1963).

    Google Scholar 

  • Lacroix, P.: Bone cartilage. In: The cell (J. Brachet and A. E. Mirsky, ed.), vol. 5, p. 219–266. New York and London: Academic Press 1961.

    Google Scholar 

  • Lafontaine, J.G., Allard, C.: A light and electron microscope study of the morphological changes induced in rat liver cells by the azo dye 2-ME-DAB. J. Cell Biol. 22, 143–172 (1964).

    Google Scholar 

  • Lehninger, A.L.: The mitochondrion. Molecular basis of structure and function. New York: W.A. Benjamin, Inc. 1964.

    Google Scholar 

  • Lennep, E.W. van, Lanzing, W.: The ultrastructure of glandular cells in the external dendritic organ of some marine catfish. J. Ultrastruct. Res. 18, 333–344 (1967).

    Google Scholar 

  • Luft, J.H.: Permanganate — a new fixative for electron microscopy. J. biophys. biochem. Cytol. 2, 799–801 (1956).

    Google Scholar 

  • Marks, B.H., Alpert, M., Kruger, F.A.: Effect of amphenone upon steroid-genesis in the adrenal cortex of golden hamster. Endocrinology 63, 75–81 (1958).

    Google Scholar 

  • Mollenhauer, H.H., Zebrun, W.: Permanganate fixation of the Golgi complex and other cytoplasmic structures of mammalian testis. J. biophys. biochem. Cytol. 8, 761–774 (1960).

    Google Scholar 

  • Philpott, C.W., Copeland, D.E.: Fine structure of chloride cells from three species of Fundulus. J. Cell Biol. 18, 389–404 (1963).

    Google Scholar 

  • Porter, K.R., Yamada, E.: Studies on the endoplasmic reticulum. V. Its form and differentiation in pigment epithelial cells of the frog retina. J. biophys. biochem. Cytol. 8, 181–205 (1960).

    Google Scholar 

  • Schmitt, F.O., Gross, J., Highberger, J.H.: Tropocollagen and the properties of fibrous collagen. Exp. Cell Res., Suppl. 3, 326–333 (1955).

    Google Scholar 

  • Sedar, A.W.: Electron microscopy of the oxyntic cell in the gastric glands of the bullfrog, Rana catesbiana. III. Permanganate fixation of the endoplasmic reticulum. J. Cell Biol. 14, 152–156 (1962).

    Google Scholar 

  • Sjöstrand, F.S.: Molecular structure of cytoplasmic membranes and of mitochondria. In: Intracellular membranous structure (S. Seno and E.V. Cowdry, ed.), p. 103–125. Okayama, Japan: Chugoku Press 1964.

    Google Scholar 

  • Slautterback, D.B.: Mitochondria in cardiac muscle cells of the canary and some other birds. J. Cell Biol. 24, 1–29 (1965).

    Google Scholar 

  • Wassermann, F., Lindenbaum, A.: Experiments concerning the effect of enzymes on the reconstitution of collagenous fibrils in vitro. J. biophys. biochem. Cytol., Suppl. 2, 299–302 (1956).

    Google Scholar 

  • Yamada, E.: Some observations on the fine structure of the interstitial cells in the human testis. In: Fifth Internat. Congr. for Electron Microscopy in Philadelphia (S.S. Breese, Jr., ed.), vol. 2, LL-1. New York and London: Academic Press 1962.

    Google Scholar 

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This investigation was supported by grants from the Swedish Natural Science Research Council, which are gratefully acknowledged. The author wishes to thank Mrs. I. Rehnberg for technical assistance.

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Abolinš-Krogis, A. Alterations in the fine structure of cytoplasmic organelles in the hepatopancreatic cells of shell-regenerating snail, Helix pomatia L.. Z. Zellforsch. 108, 516–529 (1970). https://doi.org/10.1007/BF00339657

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