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Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata

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Summary

Chloroplast tranfer was achieved by protoplast fusion between Nicotiana tobacum (Cestreae, Cestroideae) and Salpiglossis sinuata (Salpiglossideae, Cestroideae) in the family Solanaceae. Isolation of cybrid clones was facilitated by irradiation of the cytoplasm donor protoplasts, and the use of appropriate plastid mutants, streptomycin-resistant as donor, or light-sensitive as recipient. Cybrid colonies were selected by their green colour against the background of bleached (light-sensitive or streptomycin-sensitive) colonies. In the Nicotiana (Salpiglossis) cybrid plants possessing normal tobacco morphology and chromsome number, the presence of Salpiglossis, plastids was verified by restriction analysis of the chloroplast DNA. A similar analysis of the mitochondrial DNA of these lines revealed unique, recombinant patterns in the case of both fertile and sterile plants. Progeny showed no appearance of chlorophyll-deficiency in F1 and an additional back-cross generation. Attempts at transfer of entire chloroplasts between Nicotiana tabacum and Solanum nigrum (Solaneae, Solanoideae) did not result in any cybrid cell lines in a medium suitable for green colony formation of both species. These results suggest that fusion-mediated chloroplast transfer can surmount a considerable taxonomical distance, but might be hampered by a plastome-genome incompatibility in more remote combinations.

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Author notes

  1. Nguyen Duc Thanh

    Present address: Laboratory of Cell Biology, Institute of Biology, National Scientific Research Center, Nghia do, Tu liem, Hanoi, Vietnam

  2. Marvin A. Smith

    Present address: Graduate Section of Biochemistry, Brigham Young University, 84602, Provo, UT, USA

Authors and Affiliations

  1. Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    Nguyen Duc Thanh, Anikó Páy, Marvin A. Smith, Péter Medgyesy & László Márton

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  1. Nguyen Duc Thanh
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  2. Anikó Páy
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  3. Marvin A. Smith
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  4. Péter Medgyesy
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  5. László Márton
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Communicated by R.G. Herrmann

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Thanh, N.D., Páy, A., Smith, M.A. et al. Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata . Mol Gen Genet 213, 186–190 (1988). https://doi.org/10.1007/BF00339580

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  • DOI: https://doi.org/10.1007/BF00339580

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