Summary
A modified PAS-methyl blue staining method is described which, in conjunction with aldehyde fuchsin, enables the tinctorial differentiation of two types of gonadotrophs in the pituitary gland of the rat. That these two cell types are both gonadotrophic in function is indicated by their development into distinct PAS-red and PAS-purple castration cells following gonadectomy. In addition to these two kinds of castration cells, thyroidectomy cells which are PAS-negative, aldehyde fuchsin-negative, and have an affinity for orange G are seen in the pituitary glands of animals that have been both gonadectomized and thyroidectomized. Thus, in such glands the three types of modified basophiles are tinctorially different. Differences in the distribution of the PAS-red and PAS-purple gonadotrophs are characteristic of the glands of normal animals. Following gonadectomy, however, extensive shifts in the distribution of these cells occur so that the normal pattern is soon lost.
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This study was supported by research grants B-410 and RG-4723 from the U. S. Public Health Service.
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Rennels, E.G. Two tinctorial types of gonadotrophic cells in the rat hypophysis . Zeitschrift für Zellforschung 45, 464–471 (1957). https://doi.org/10.1007/BF00338888
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DOI: https://doi.org/10.1007/BF00338888