Summary
Methods for isolation of hybrids of industrial yeast strains, obtained by PEG-mediated protoplast fusion, using mitochondrial mutations to antibiotic resistance and to the petite condition as markers, are described. One of the industrial prototrophic strains, carrying a rescuable mutation to antibiotic resistance, was converted to the petite form, and protoplasts obtained from it were fused with protoplasts from antibiotic-sensitive prototrophic strains of brewing and distiller's yeasts or with an auxotrophic laboratory strain carrying several chromosomal mutations. The parent of the petite strain was also able to metabolize starch, which was used as an additional character for confirming the hybrid nature of the strains isolated on the basis of their antibiotic resistance.
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Communicated by F. Kaudewitz
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Spencer, J.F.T., Laud, P. & Spencer, D.M. The use of mitochondrial mutants in the isolation of hybrids involving industrial yeast strains. Molec. gen. Genet. 178, 651–654 (1980). https://doi.org/10.1007/BF00337874
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DOI: https://doi.org/10.1007/BF00337874