Summary
A method of hemoproteid-deficient mutants isolation is described. 13 mutants lacking catalase belonging to 9 complementation groups were isolated. Mutants representing 7 loci were also unable to form cytochromes.
Addition of δ-aminolevulinic acid does not restore catalase activity. Normal mendelian segregation in crosses with wild strain was observed only in three mutants. The rest give 4:0 segregation. After ethidium bromide treatment and induction of rho — strains normal segregation was observed in all mutants. The masking effect of normal mitochondrial activity on gene expression in mutant strains is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avers, C. J., Federman, J.: The occurrence in yeast of cytoplasmic granules which resemble microbodies. J. Cell Biol. 37, 555–559 (1968)
Biliński, T., Kotylak, Z., Jachymczyk, W.: The dependence of cytosole protein biosynthesis resistance to cycloheximide in yeast on changes in mitochondrial activity. Molec. gen. Genet. 129, 243–248 (1974)
Labbe, P.: Synthèse du protoheme par la levure Saccharomyces cerevisiae. Biochemie 53, 1001–1014 (1971)
Lachowicz, T. M., Kotylak, Z., Kolodyński, J., Sniegocka, Z.: New types of respiratory deficient mutants in Saccharomyces cerevisiae. II. Physiology and genetics of a series of segregational mutants induced by ultraviolet irradiation or nitrous acid treatment. Arch. Immunol et Ther. Exp. (Wrocŀaw) 17, 72–85 (1969)
McKay, R., Druyan, R., Getz, G. S., Rabinowitz, M.: Intramitochondrial localisation of δ-aminolevulinate synthetase and ferrochelatase in rat liver. Biochem. J. 114, 455–461 (1969)
Pachecka, J., Sitkiewicz, D.: Correlation between the respiratory enzymes and catalase synthesis in yeast. Bull. Acad. Polon. Sci., Ser. biol. 19, 17–21 (1971)
Puglisi, P. P., Algeri, A.: Role of the mitochondrion in the regulation of protein synthesis in the eucaryote Saccharomyces cerevisiae. Molec. gen. Genet. 110, 110–118 (1971)
Raut, C.: A cytochrome-deficient mutant of Saccharomyces cerevisiae. Exp. Cell Res.4, 295–305 (1953)
Rogers, P. J., Stewart, P. R.: Respiratory development in Saccharomyces cerevisiae grown at controlled oxygen tension. J. Bact. 115, 88–97 (1973)
Sanders, H. K., Mied, P. A., Briquet, M., Hernandez-Rodriguez, J., Gottal, R. F., Matoon, J. R.: Regulation of mitochondrial biogenesis: Yeast mutants deficient in synthesis of δ-aminolevulinic acid. J. molec. Biol. 80, 17–39 (1973)
Sano, S., Granick, S.: Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation. J. biol. Chem. 236, 1173–1180 (1961)
Serrano, R., Gancedo, J. M., Gancedo, C.: Assay of yeast enzymes in situ. A potential tool in regulation studies. Europ. J. Biochem. 34, 479–482 (1973)
Sherman, F.: Mutants of yeast deficient in cytochrome c. Genetics 49, 39–49 (1964)
Sugimura, T., Okabe, K., Nagao, M., Gunge, N.: A respiration-deficient mutant of Saccharomyces cerevisiae which accumulates porhyrins and lack cytochromes. Biochim. biophys. Acta (Amst.) 115, 267–275 (1966)
Sumner, J. B., Dounce, A. L.: In: Methods in enzymology II, p. 775 (Colowick, S. P., Kaplan, N. O., eds), New York: Academic Press 1955
Ycas, M., Starr, T. J.: The effect of glycine and protoporphyrin on cytochrome deficient yeast. J. Bact. 65, 83–88 (1953)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Pachecka, J., Litwińska, J. & Biliński, T. Hemoproteid formation in yeast. Molec. gen. Genet. 134, 299–305 (1974). https://doi.org/10.1007/BF00337464
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337464