Abstract
The isolation of organelles devoid of other cellular compartments is crucial for studying organellar proteomes and the localization of newly identified proteins, as well as for assessing specific organellar functions. Here, we describe a protocol for the isolation of crude and highly pure mitochondria from Saccharomyces cerevisiae and provide methods for testing the functional integrity of the isolated organelles.
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Acknowledgments
Our work is supported by the Deutsche Forschungsgemeinschaft (DFG) under Germany’s Excellence Strategy (CIBSS – EXC-2189 – Project ID 390939984 to C.M., F.N.V. and A.A.T.), the RTG 2202/278002225 (to C.M.), RTG 2206/423813989 (to C.M. and F.N.V.), the SFB 1381 (Project-ID 403222702, to C.M. and F.N.V.), and the Emmy-Noether program (to F.N.V).
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Taskin, A.A., Moretti, D.N., Vögtle, F.N., Meisinger, C. (2023). Isolation and Quality Control of Yeast Mitochondria. In: Nicholls, T.J., Uhler, J.P., Falkenberg, M. (eds) Mitochondrial DNA. Methods in Molecular Biology, vol 2615. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2922-2_4
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DOI: https://doi.org/10.1007/978-1-0716-2922-2_4
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Online ISBN: 978-1-0716-2922-2
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