Summary
The spermatozoa of Peregrinus maidis Ashm. are thread-like, approximately 650 μ long and 1 μ wide including the head (approximately 28 μ).
The main part of the spermatozoa consists of two mitochondria derivatives, a central body between them, the axial filament complex, and a newly found element consisting of two wing-shaped bodies. Each mitochondrion derivative shows a peripheral and an inner part. The peripheral part is formed by cristae arranged perpendicularly to the long axis of the spermatozoon. The cristae are approximately 70 Å wide. The dense layers between them measure approximately 280 Å. The inner part of the mitochondrion derivative shows a crystalline array, formed by sub-units of approximately 100 Å diameter. The wing-shaped bodies consist of tubular elements.
The head has an elongated nucleus with an electron transparent space inside. At the anterior end of the nucleus lies a tapered acrosome. This appears fibrous and parts of the acrosome fibers seem to run along the nucleus.
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André, J.: Sur quelques détails nouvellement connus de l'ultrastructure des organites vibratiles. J. Ultrastruct. Res. 5, 86–108 (1961).
Barth, R.: Métodos usados en microanatomía e histología entomologica. Mem. Inst. Osw. Cruz 56, 467 (1958).
Bawa, S. R.: Electron microscope study of spermiogenesis in a fire-brat insect, Thermobia domestica Pack. I. Mature spermatozoon. J. Cell Biol. 23, 431–446 (1964).
Burgos, M. H., and D. W. Fawcett: Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J. biophys. biochem. Cytol. 1, 287–300 (1955).
—, and D. W. Fawcett: An electron microscope study of spermatid differentiation in the toad, Bufo arenarum Hensel. J. biophys. biochem. Cytol. 2, 223–240 (1956).
Cameron, M. L.: Some details of ultrastructure in the development of flagellar fibers of the Tenebrio sperm. Canad. J. Zool. 43, 1005–1010 (1965).
Fawcet, D. W.: The structure of the mammalian spermatozoon. Int. Rev. Cytol. 7, 195–234 (1958).
—: In: The cell. vol. II, Cilia and Flagella, p. 217–297. New York and London: Academic Press 1961.
Galtsoff, P. S., and D. E. Philpott: Ultrastructure of the oyster, Crassostrea virginica. J. Ultrastruct. Res. 3, 241–253 (1960).
Kaye, J. S.: Changes in the fine structure of mitochondria during spermatogenesis. J. Morph. 102, 347–399 (1958).
—: The fine structure of flagella in spermatids of the house cricket. J. Cell Biol. 22, 710–714 (1964).
Meyer, G. F.: Die parakristallinen Körper in den Spermienschwänzen von Drosophila. Z. Zellforsch. 62, 762–784 (1964).
—: Crystalline mitochondrial derivatives in sperm. Sixth Int. Congr. Electron Microscopy, Kyoto, vol. II, p. 629–630. Tokyo: Maruzen Co., Ltd. 1966.
Millonig, G.: A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol. 11, 736–739 (1961).
Nagano, T.: Observations on the fine structure of the developing spermatid in the domestic chicken. J. Cell Biol. 14, 193–205 (1962).
Rebhun, L. I.: Nuclear changes during spermiogenesis in a pulmonate snail. J. biophys. biochem. Cytol. 3, 509–524 (1957).
Reger, J. F.: A study on the fine structure of developing spermatozoa from the isopod, Asellus militaris (Hay). J. Microscopie 3, 559–572 (1964).
Roth, L. E.: A filamentous component of protozoan fibrillar systems. J. Ultrastruct. Res. 1, 223–234 (1958).
Sjöstrand, F. S., and B. A. Afzelius: Electron microscopy on grasshopper spermatids. Electron microscopy, Proc. of the Stockholm Conference, p. 164–167. New York and London: Academic Press 1957.
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).
Werner, G.: Untersuchungen über die Spermiogenese beim Sandläufer, Cincindela campestris L. Z. Zellforsch. 66, 255–275 (1965).
—: Untersuchungen über die Spermiogenese bei einem Laufkäfer, Carabus catenulatus Scop. und der Skorpion-Wasserwanze, Nepa rubra L. Z. Zellforsch. 73, 576–599 (1966).
Yasuzümi, G., W. Fujimura, and H. Ishida: Spermatogenesis in animal as revealed by electron microscopy. V. Spermatid differentiation of Drosophila and grasshopper. Exp. Cell Res. 14, 268–385 (1958).
—, and H. Tanaka: Spermatogenesis in animals as revealed by electron microscopy. VI. Researches on the spermatozoon — dimorphism in a pond snail Cipangopaludina malleata. J. biophys. biochem. Cytol. 4, 621–632 (1958).
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Acknowledgements. The authors wish to thank Dr. G. H. Bergold for suggestions and support, Drs. J. André, D. W. Fawcett, P. Maillet and G. F. Meyer for very helpful discussion. They are also grateful to Mr. O. Suárez for assistance in the preparation of the organs of P. maidis and to Mrs. M. de Pingarrón for technical assistance.
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Herold, F., Munz, K. Ultrastructure of spermatozoa of Peregrinus maidis (Homoptera, Delphacidae). Zeitschrift für Zellforschung 83, 364–374 (1967). https://doi.org/10.1007/BF00336865
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DOI: https://doi.org/10.1007/BF00336865