Skip to main content
SpringerLink
Log in

Influence of soluble phosphorus fertilizer on the interaction between a vesicular-arbuscular mycorrhizal fungus and Azospirillum brasilense in barley (Herdeum vulgare L.)

  • Published:
Biology and Fertility of Soils Aims and scope Submit manuscript

Summary

Pot-culture studies were carried out to examine the response of barley (Hordeum vulgare L.) to inoculation with Azospirillum brasilense and Glomus versiforme, singly and/or in combination, under varying levels of nitrogenous [(15NH4)2SO4] and soluble phosphatic (single superphosphate) fertilizers. The interaction between both the endophytes led to increased growth and nutrition of the barley plants. Roots from plants inoculated with Azospirillum brasilense and Glomus versiforme exhibited very low acetylene reduction activity. N2 fixation in the plants increased with the increase in plant growth but the mycorrhiza alone gave a low level of N2 fixation in the plants compared to combined inoculation with both the endophytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alexander M (1965) Most probable number method for microbial populations. In: Black CA et al. (eds) Methods of soil analysis, part II. pp 1467–1472

  • Azcon R, Ocampo JA (1981) Factors effecting the VAM infection and mycorrhizal dependency of thirteen wheat cultivars. New Phytol 87:677–685

    Google Scholar 

  • Barea JM, Boris AF, Olivers J (1983) Interactions between Azospirillum and VA-mycorrhizae and their effects on growth and nutrition of maize and rye grass. Soil Biol Biochem 15:705–709

    Google Scholar 

  • Bierman B, Lindermann RG (1981) Quantifying VA-mycorrhizae: A proposed method towards standardization. New Phytol 87:63–67

    Google Scholar 

  • Bouton JH, Smith RL, Schank SC, Bouton CW, Tyler ME, Littell RC, Gallaher RN, Quessenberry KH (1979) Response of pearl millet inbreds and hybrids to inoculation with Azospirillum brasilense. Crop Sci 19:12–16

    Google Scholar 

  • Gerdemann JW (1968) Vesicular-arbuscular mycorrhiza and plant growth. Annu Rev Phytopathol 6:379–418

    Google Scholar 

  • Hardy RWF, Holsten RD, Jackson EK, Burns RC (1968) The acetyleneethylene assay for nitrogen fixation—laboratory and field evaluation. Plant Physiol 43:1185–1207

    Google Scholar 

  • Hetrick BAD, Bockus WW, Bloom J (1984) Role of VAM fungi in the growth of Kansas winter wheat. Can J Bot 62:735–740

    Google Scholar 

  • Neyra CA, Döbereiner J (1977) Nitrogen fixation in grasses. Adv Agron 29:1–38

    Google Scholar 

  • O'Hara GW, Davey NR, Lucas JA (1981) Effect of inoculation of Zea mays with Azospirillum brasilense strains under temperate conditions. Can J Microbiol 27:871–877

    Google Scholar 

  • Pacovski RS, Fuller G (1985) Influence of soil on the interaction between endomycorrhizae and Azospirillum in sorghum. Soil Biol Biochem 17:525–535

    Google Scholar 

  • Pacovski RS, Paul EA, Bethlenfalvay GJ (1986) Response of mycorrhizal and P fertilized soybeans to nodulation by Bradyrhizobium and ammonium nitrate. Crop Sci 26:145–150

    Google Scholar 

  • Panse VG, Sukhatme PV (1954) Statistical methods for agricultural workers. Publications and Information Division, Indian Council of Agricultural Research, New Delhi

    Google Scholar 

  • Philips JM, Hayman DS (1970) Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161

    Google Scholar 

  • Seiverding E (1983) Influence of soil water regime on VA-mycorrhiza. II. Effect of soil temperature and water regime on growth, nutrient uptake and water utilization of Eupatorium odoratum. Z Acker Pflanzenbau 152:56–67

    Google Scholar 

  • Subba Rao NS, Tilak KVBR, Singh CS (1985a) Effect of combined inoculation of VAM and Azospirillum brasilense on pearl millet (Penisetum americanum). Plant and Soil 84:283–286

    Google Scholar 

  • Subba Rao NS, Tilak KVBR, Singh CS (1985b) Synergistic effects of vesicular-arbuscular mycorrhizae and Azospirillum brasilense on the growth of barley plants in pots. Soil Biol Biochem 17:119–121

    Google Scholar 

  • Tien TM, Gaskin MH, Hubbell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Penisetum americanum L.). Appl Environ Microbiol 37:1016–1024

    Google Scholar 

  • Venkateswarlu B, Rao AV (1983) Response of pearl millet to inoculation with different strains of Azospirillum brasilense. Plant and Soil 74:379–386

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Microbiology, Indian Agricultural Research Institute, 110012, New Delhi, India

    Mahima Negi & K. V. B. R. Tilak

  2. Nuclear Research Laboratory, Indian Agricultural Research Institute, 110012, New Delhi, India

    M. S. Sachdev

Authors
  1. Mahima Negi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. S. Sachdev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. V. B. R. Tilak
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Negi, M., Sachdev, M.S. & Tilak, K.V.B.R. Influence of soluble phosphorus fertilizer on the interaction between a vesicular-arbuscular mycorrhizal fungus and Azospirillum brasilense in barley (Herdeum vulgare L.). Biol Fertil Soils 10, 57–60 (1990). https://doi.org/10.1007/BF00336125

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00336125

Key words

Search

Navigation