Summary
In the dorsal root ganglia of the rat, ribosomes were found not only in the initial segment, but they were also observed in the axoplasm of intraganglionar myelinated fibres and in the sensory portion of spinal nerves. Axons of seven-days-old rats contained more ribosomes than those of adult animals. The amount of particles decreased gradually from the initial segment trough intraganglionar internodes to the axons of spinal nerves. No ribosomes were found in axons of dorsal roots. In intraganglionar fibres, ribosomal particles were usually observed near the nodes of Ranvier, in the vicinity of Schmidt-Lantermann clefts and in axons near the Schwann cell nuclei. They were arranged in tetrads, pentads or in larger polysomes, and they were often observed adjacent to a group of mitochondria.
The particles had invariably a stable size, their average diameters measuring 234 ± 2 × 197 ± 3 Å, which is practically equal to the diameters of 232 ± 2 × 203 ± 3 Å of ribosomes in the Schwann cell cytoplasm. These values fall within the range of diameters of ribosomes isolated from various cells of eukaryotic organisms as given in the literature. Since no other granular component of the cytoplasm has similarly stable dimensions, the measurements are considered to prove that the axonal particles described here are ribosomes.
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References
Amaldi, P., Rusca, G.: Autoradiographic study of RNA in nerve fibers of embryonic sensory ganglia cultured in vitro under NGF stimulation. J. Neurochem. 17, 767–771 (1970).
André, J., Marinozzi, V.: Présence dans les mitochondries de particules ressemblant aux ribosomes. J. Microsc. 4, 615–626 (1965).
Andres, K. H.: Untersuchungen über den Feinbau von Spinalganglien. Z. Zellforsch. 55, 1–48 (1961).
Austin, L., Morgan, I. G., Bray, J. J.: The biosynthesis of proteins within axons and synaptosomes. In: Protein metabolism of the nervous system (A. Lajtha, ed.) p. 271–289. New York-London: Plenum Press 1970.
Barondes, S. H., ed.: Cellular dynamics of the neuron, 383 p. Symp. of the Internat. Soc. for Cell Biology, vol. 8. New York: Academic Press 1969.
- Samson F. E., Jr., eds.: Axoplasmic transport. Neurosciences Res. Progr. Bull. 5, 307–419 (1967).
Beattie, D. S., Basford, R. E., Koritz, S. B.: The turnover of the protein components of mitochondria from rat liver, kidney and brain. J. biol. Chem. 242, 4584–4586 (1967).
Bray, J. J., Austin, L.: Flow of protein and ribonucleic acid in peripheral nerve. J. Neurochem. 15, 731–740 (1968).
Caley, D. W., Maxwell, D. S.: An electron microscopic study of neurons during postnatal development of the rat cerebral cortex. J. comp. Neurol. 133, 17–28 (1968).
Conradi, S.: Ultrastructural specialization in the initial axon segment of cat lumbar motoneurons. Acta Soc. Med. upsalien. 71, 281–284 (1966).
Curgy, J. J.: Incorporation in vivo et in vitro d'uridine-3H dans l'ARN des mitochondries. Exp. Cell Res. 62, 359–367 (1970).
Droz, B., Koenig, H. L.: Localization of protein metabolism in neurons. In: Protein metabolism of the nervous system (A. Lajtha, ed.) p. 93–108. New York-London: Plenum Press 1970.
Edström, A.: RNA protein synthesis in Mauthner nerve fiber components of fish. In: Cellular dynamics of the neuron, ed. S. H. Barondes. Symp. of the Internat. Soc. for Cell Biology, vol. 8, p. 95–111. New York: Academic Press 1969.
Edström, J.-E., Eichner, D., Edström, A.: The ribonucleic acid of axons and myelin sheaths from Mauthner neurons. Biochim. biophys. Acta (Amst.) 61, 178–184 (1962).
Fischer, J., Blinzinger, K.: Vorkommen von Glykogen in geschwollenen Dendriten bei experimenteller Kobaltnekrose des Rattengehirns. Virchows Arch. Abt. B 1, 201–210 (1968).
Fischer, S., Cellino, M., Gariglio, P., Tellez-Nagel, I.: Protein and RNA metabolism of squid axons (Dosidicus gigas). J. gen. Physiol. 51, 72s-80s (1968).
Gray, E. G.: Electron microscopy of presynaptic organelles of the spinal cord. J. Anat. (Lond.) 97, 101–106 (1963).
Ha, H.: Axonal bifurcation in the dorsal root ganglion of the cat: a light and electron microscope study. J. comp. Neurol. 140, 227–240 (1970).
Haldar, D., Freeman, K. B., Work, T. S.: The site of synthesis of mitochondrial proteins in Krebs II ascites—tumor cells. Biochem. J. 102, 684–690 (1967).
Hughes, A.: The development of the neural tube of the chick embryo. A study with the ultraviolet microscope. J. Embryol. exp. Morph. 3, 305–325 (1955).
Jakoubek, E., Edström, J.-E.: RNA changes in the Mauthner axon and myelin sheath after increased functional activity. J. Neurochem. 11, 845–849 (1965).
Jirmanová, I.: Glycogen-deposits in motoneurones of young chickens following peripheral nerve section. J. Neuropath. exp. Neurol. (in press).
Kadenbach, B.: Synthesis of mitochondrial proteins: Demonstration of a transfer of proteins from ribosomes into mitochondria. Biochim. biophys. Acta 134, 430–442 (1966).
Koenig, E.: Synthetic mechanisms in the axon. II. RNA in myelin-free axons of the cat. J. Neurochem. 12, 357–361 (1965).
—: Synthetic mechanisms in the axon. IV. In vitro incorporation of (3H) precursors into axonal protein and RNA. J. Neurochem. 14, 437–446 (1967).
—: The axon as a heuristic model for studying membrane protein-synthesizing machinery. In: Protein metabolism of the nervous system (A. Lajtha, ed.) p. 259–269. New York-London: Plenum Press 1970.
Lubińska, L.: Axoplasmic streaming in regenerating and in normal nerve fibres. In: Mechanisms of neural regeneration (M. Singer J. P. Schadé, eds.) Progress in brain research, vol. 13, p. 1–71. Amsterdam: Elsevier 1964.
Lyser, K. M.: Early differentiation of motor neuroblasts in the chick embryo as studied by electron microscopy. I. General aspects. Develop. Biol. 10, 433–466 (1964).
—: Early differentiation of motor neuroblasts in the chick embryo as studied by electron microscopy. II. Microtubules and neurofilaments. Develop. Biol. 17, 117–142 (1968).
Masurovsky, E., Bunge, M. B., Bunge, R. P.: Cytological studies of organotypic cultures of rat dorsal root ganglia following X-irradiation in vitro. I. Changes in neurons and satellite cells. J. Cell Biol. 32, 467–496 (1967).
Miani, N., Girolamo, A, di, Girolamo, M., di: Sedimentation characteristics of axonal RNA in rabbit. J. Neurochem. 13, 755–759 (1966).
Mugnaini, E., Forstrønen, P. F.: Ultrastructural studies on the cerebellar histogenesis. I. Differentiation of the granule cells and development of glomeruli in the chick embryo. Z. Zellforsch. 77, 115–143 (1967).
—, Walberg, F., Hauglie-Hanssen, E.: Observations on the fine structure of the lateral vestibular nucleus (Deiters' nucleus) in the cat. Exp. Brain Res. 4, 146–186 (1967).
Palay, S. L., Palade, G. E.: The fine structure of neurons. J. biophys. biochem. Cytol. 1, 69–88 (1955).
—, Sotelo, C., Peters, A., Orkand, P. M.: The axon hillock and the initial segment. J. Cell Biol. 38, 193–201 (1968).
Peters, A., Palay, S. L., Webster, H. de F.: The fine structure of the nervous system, 198 p. New York: Harper and Row 1970.
—, Proskauer, C. G., Kaiserman-Abramof, I. R.: The small pyramidal neuron of the cat cerebral cortex. The axon hillock and the initial segment. J. Cell Biol. 39, 604–619 (1968).
Peterson, J. A., Bray, J. J., Austin, L.: An autoradiographic study of the flow of protein and RNA along peripheral nerve. J. Neurochem. 15, 741–745 (1968).
Pomerat, C. M., Hendelman, W. J., Rainborn, Ch. W., Jr., Massey, J. F.: Dynamic activities of nervous tissue in vitro. In: The neuron (H. Hydén, ed.), p. 119–178. Amsterdam: Elsevier 1967.
Rahmann, H.: Zum Stofftransport im Zentralnervensystem der Vertebraten. Autoradiographische Untersuchungen mit P-32-Orthophosphat, H-3-Histidin, H-3-Uridin an Mäusen und Fischen. Z. Zellforsch. 66, 878–890 (1965).
Rich, A.: The ribosome: a biological information translator. In: The Neurosciences (G. C. Quarton, T. Melnechuk, F. O. Schmitt, eds.), p. 101–112. New York: Rockefeller University Press 1967.
Roodyn, D. B.: Protein synthesis in mitochondria. 3. The controlled disruption and subfractionation of mitochondria labelled in vitro with radioactive valine. Biochem. J. 85, 177–189 (1962).
Roodyn, D. S., Wilkie, D.: The biogenesis of mitochondria. 123 p. London: Methuen & Co. Ltd, 1968.
Scharf, J. H.: Sensible Ganglien, 485 S. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. IV/3 (W. Bargmann, ed.). Berlin-Göttingen-Heidelberg: Springer 1958.
Singer, M., Green, M. R.: Autoradiographic studies of uridine incorporation in peripheral nerve of the newt, Triturus. J. Morph. 124, 321–343 (1968).
Slagel, D. E., Hartmann, H. A., Edström, J. E.: The effect of iminodipropionitrile on the ribonucleic acid content and composition of mesencephalic V cells, anterior horn cells, glial cells, and axonal balloons. J. Neuropath. exp. Neurol. 25, 244–253 (1966).
Spirin, A. S., Gavrilova, L. P.: The ribosome, 161 p. Berlin-Heidelberg-New York: Springer 1969.
Tennyson, V. M.: Electron microscopic study of the developing neuroblast of the dorsal root ganglion of the rabbit embryo. J. comp. Neurol. 124, 267–318 (1965).
—: The fine structure of the axon and growth cone of the dorsal root neuroblast of the rabbit embryo. J. Cell Biol. 44, 62–79 (1970).
Utakoji, T., Hsu, T. C.: Nucleic acid and protein synthesis of isolated cells from chick embryonic spinal ganglia in culture. J. exp. Zool. 158, 181–201 (1965).
Walberg, F.: The fine structure of the cuneate nucleus in normal cats and following interruption of afferent fibres. An electron microscopical study with particular reference to findings made in Glees and Nauta sections. Exp. Brain Res. 2, 107–128 (1966).
Watson, H. L., Aldridge, W. G.: Selective electron staining of nucleic acids. J. Histochem. Cytochem. 12, 96–103 (1964).
Zelená, J.: Bidirectional movements of mitochondria along axons of an isolated nerve segment. Z. Zellforsch. 92, 186–196 (1968).
—: Bidirectional shift of mitochondria in axons after injury. In: Cellular dynamics of the neuron (S. H. Barondes, ed.), Symp. of the Internat. Soc. for Cell Biology, vol. 8, p. 73–94. New York: Academic Press 1969.
—: Ribosome-like particles in myelinated axons of the rat. Brain Res. 24, 359–363 (1970).
- Ribosomes in the axoplasm of myelinated nerve fibres. Folia morphol., in press.
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The author wishes to thank Dr. K. Smetana for his valuable suggestions and Mrs. M. Sobotková, Ing. M. Doubek and Mr. H. Kunz for their skillful technical assistance. The investigation was in part supported by a grant-in-aid from the Muscular Dystrophy Associations of America, Inc.
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Zelená, J. Ribosomes in myelinated axons of dorsal root ganglia. Z.Zellforsch. 124, 217–229 (1972). https://doi.org/10.1007/BF00335680
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DOI: https://doi.org/10.1007/BF00335680