Summary
During a survey of liver tissue from 100 dogs, fine tubules were observed within the cisternae of the endoplasmic reticulum of hepatocytes in one dog. The tubules were 300 Å in diameter with electron dense walls of 100 Å thickness. Many cells contained the tubules but their significance is unknown. The tubules varied from other reported microtubules in their location, size and characteristics of fixation and staining.
Similar content being viewed by others
References
Behnke, O.: A preliminary report on “microtubules” in undifferentiated and differentiated vertebrate cells. J. Ultrastruct. Res. 11, 139–146 (1964).
Bruni, C., and K. R. Porter: The fine structure of the parenchymal cell of the normal rat liver. I. General observations. Amer. J. Path. 46, 691–756 (1965).
Burgos, M. H., and D. W. Fawcett: Studies on the fine structure of the mammalian testes. J. biophys. biochem. Cytol. 1, 287–300 (1955).
Caulfield, J. B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. biophys. biochem. Cytol. 3, 827–830 (1957).
De-Thé, G.: Cytoplasmic microtubules in different animals cells. J. Cell Biol. 23, 265–275 (1964).
Hepler, P. K., and E. H. Newcomb: Microtubules and fibrils in the cytoplasm of coleus cells undergoing secondary wall deposition. J. Cell Biol. 20, 529–533 (1964).
Ledbetter, M. C., and K. R. Porter: A “microtubule” in plant cell fine structure. J. Cell Biol. 19, 239–250 (1963).
—: Morphology of microtubules of plant cells. Science 144, 872–874 (1964).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–413 (1961).
Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–298 (1952).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sandborn, E., P. F. Koen, J. D. McNabb, and G. Moore: Cytoplasmic microtubules in mammalian cells. J. Ultrastruct. Res. 11, 123–138 (1964).
Slautterback, D. B.: Cytoplasmic microtubules. I. Hydra. J. Cell Biol. 18, 367–388 (1963).
Stein, R. J., W. R. Richter, S. M. Moize, and E. J. Rdzok: Comparative hepatic ultrastructure of the street dog and registered beagles. Fed. Proc. 23, 578 (1964).
Wolfe, S. L.: Isolated microtubules. J. Cell Biol. 25, 408–413 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Richter, W.R., Bischoff, M.B. & Churchill, R.A. An observation of fine tubules within the endoplasmic reticulum in a dog liver. Zeitschrift für Zellforschung 70, 180–184 (1966). https://doi.org/10.1007/BF00335672
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00335672