Abstract
The determination of benzoic, 3-and 4-methylbenzoic, and mandelic acids in urine by gas chromatography (G.C.) is presented. The analytical procedure includes: addition of internal standard (β-naphthol), alkaline hydrolysis, ethyl ether extraction, evaporation of the solvent, and silylation of acids with Silyl-8 in pyridine (1∶1).
The results are calculated from ratios of the internal standard and determined metabolites peak surface areas.
The coefficient of variation of the method in determination of mentioned acids is ±8%.
Zusammenfassung
In der Arbeit wird die Gesamtbestimmung von Benzoesäure, 3-und 4-Methylbenzoesäure sowie Mandelsäure im Harn mit der Gaschromatographie (G.C.) dargestellt. Das analytische Verfahren umfaßt die Addition des inneren Standards β-Naphthol zur Harnprobe, die alkalische Hydrolyse, die Harnextraktion mit Äthyläther, das Lösungsmittelabdämpfen sowie die Silylierung der trockenen Rückstände (von Säuren) mit dem Gemisch Silyl-8-Pyridin (1 ∶ 1).
Die Ergebnisse werden anhand der Relationen aus den Peakflächen der zu bestimmenden Metaboliten und dem inneren Standard (Standardabweichung) berechnet.
Die Genauigkeit der erarbeiteten Methode beträgt bei der Bestimmung der genannten Säuren ±8%.
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Wieczorek, H., Trojanowska, B. & Dramiński, W. Determination of mixtures of urinary benzoic, 3- and 4-methylbenzoic, and mandelic acids by gas chromatography. Arch Toxicol 42, 281–287 (1979). https://doi.org/10.1007/BF00334842
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DOI: https://doi.org/10.1007/BF00334842