Summary
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1.
The distribution of cholinesterase in the parotid salivary gland has been studied histochemically in 21 mature rats.
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2.
A large amount of true and pseudo cholinesterase activity was found to be present throughout the cytoplasm of the secretory cells.
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3.
Cholinesterase activity was also seen in moderate amounts in the nerve trunks accompanying the inter- and intralobular ducts and blood vessels.
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4.
Postganglionic sympathectomy was found to have no effect on the activity of cholinesterase in the secretory cells and nerve trunks.
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5.
The significance of these findings is discussed.
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References
Alles, G. A., and R. C. Hawes: Cholinesterases in the blood of man. J. biol. Chem. 133, 375–390 (1940).
Babkin, B. P., A. Alley and G. W. Stavraky: Humoral transmission of chorda tympani effect. Trans roy. Soc. Can., Sect. V 26, 89 (1932).
Babkin, B. P., O. S. Gibbs and H. G. Wolff: Die humorale Übertragung der Chorda tympani-Reizung. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 168, 32–37 (1932).
Briscoe, S.: Cholinesterase activity in bull semen. Fed. Proc. 14, 17–18 (1955).
Burn, J. H.: A discussion on the action of local hormones. Proc. roy. Soc. B 137, 281–320 (1950).
Cattell, M., H. G. Wolff and D. Clark: The liberation of adrenergic and cholinergic substances in the submaxillary gland. Amer. J. Physiol. 109, 375–385 (1934).
Dumont, L.: Localisations histochemiques d'acétylcholinestérase dans la rate et les ganglions lymphatiques. C. R. Soc. biol. (Paris) 149, 960–964 (1955a).
—: Localisation histochimique d'acétylcholinestérase dans la thyroide de lapin. C. R. Acad. Sci. (Paris) 240, 1946–1948 (1955b).
Feldberg, W., and J. A. Guimarais: Some observations on salivary secretion. J. Physiol. (Lond.) 85, 15–36 (1935).
Glick, D.: Studies on enzymatic histochemistry. XXV. A micro method for the determination of choline esterase and the activity-pH relationship of this enzyme. J. gen. Physiol. 21, 289–295 (1938).
Hebb, C., and K. J. Hill: Distribution of cholinesterases in the mammalian pancreas. Quart. J. Exp. Physiol. 40, 168–175 (1955).
Hokin, L. E., and M. R. Hokin: Metabolism of phospholipids in vitro. Canad. J. Biochem. 34, 349–360 (1956).
Koelle, G. B.: The elimination of enzymatic diffusion artifacts in the histochemical localisation of cholinesterases and a survey of their cellular distributions. J. Pharmacol. exp. Ther. 103, 153–171 (1951).
—: The histochemical identification of acetyl-cholinesterase in cholinergic, adrenergic and sensory neurons. J. Pharmacol. exper. Ther. 114, 167–184 (1955).
Koelle, G. B., and J. S. Friedenwald: A histochemical method for localising cholinesterase activity. Proc. Soc. exp. Biol. (N. Y.) 70, 617–622 (1949).
—, and J. S. nFriedenwald: The effect of inhibition of specific and nonspecific cholinesterase on the motility of the isolated ileum. J. Pharmacol. exp. Ther. 100, 180–191 (1950).
Mazur, A., and O. Bodansky: The mechanism of in vitro and in vivo inhibition of cholinesterase activity by diisopropylfluorophosphate. J. biol. Chem. 163, 261–276 (1946).
Mendel, B., and H. Rudney: Studies on cholinesterase. I. Cholinesterase and pseudo-cholinesterase. Biochem. J. 37, 59–63 (1943).
Ord, M. G., and R. H. S. Thompson: The distribution of Cholinesterase types in mammalian tissues. Biochem. J. 46, 346–352 (1950).
Secker, J.: The humoral control of the secretion by the submaxillary gland of the cat following chorda stimulation. J. Physiol. (Lond.) 81, 81–92 (1934a).
—: The humoral control of the secretion by the submaxillary gland of the cat following sympathetic stimulation. J. Physiol. (Lond.) 82, 293–304 (1934b).
Snell, R. S.: Histochemical appearances of cholinesterase in the normal sciatic nerve and the changes which occur after nerve section. Nature (Lond.) 180, 378–379 (1957a).
—: Changes in the histochemical appearances of cholinesterase in a mixed peripheral nerve following nerve section and compression injury. J. exp. Path. 38, 479–482 (1957b).
—: The histochemical appearance of cholinesterase in the superior cervical sympathetic ganglion. J. Anat. (Lond.) 91, 597–598 (1957c).
—: The histochemical appearances of cholinesterase in the superior cervical sympathetic ganglion and the changes which occur after preganglionic nerve section. J. Anat. (Lond.) 92, 408–418 (1958a).
—: The histochemical appearances of cholinesterase in the parasympathetic nerves supplying the submandibular and sublingual salivary glands of the rat. J. Anat. (Lond.) 92, 534–543 (1958b).
Snell, R. S., and J. R. Garrett: The distribution of cholinesterase in the submaxillary and sublingual salivary glands of the rat. J. Histochem. Cytochem. 5, 236–245 (1957).
—, and J. R. Garrett: The effect of postganglionic sympathectomy on the histochemical appearances of cholinesterase in the nerves supplying the submandibular and sublingual salivary glands of the rat. Z. Zellforsch. 48, 201–214 (1958).
Zajicek, J., B. Sylven and N. Datta: Attempts to demonstrate acetylcholinesterase activity in blood and bone marrow cells by a modified thiocholine technique. J. Histochem. Cytochem. 2, 115–121 (1954).
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Snell, R.S. The histochemical appearances of cholinesterase in the parotid salivary gland of the rat. Z. Zellforsch. 49, 330–338 (1959). https://doi.org/10.1007/BF00334731
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DOI: https://doi.org/10.1007/BF00334731