Abstract
In connection with testicular effects in rats of dibutyl phthalate (DBP) and monobutyl phthalate (MBP), the in vitro hydrolysis of dibutyl esters of phthalate isomers such as isophthalic and terephthalic acids was compared using preparations from rat liver, kidney, pancreas, small intestine, and blood. The results suggest considerable substrate specificity and the participation of different enzymes for hydrolysis of these esters.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- DBP:
-
di-n-butyl phthalate
- MBP:
-
mono-n-butyl phthalate
- DBIP:
-
di-n-butyl isophthalate
- DBTP:
-
di-n-butyl tere-phthalate
- MBIP:
-
mono-n-butyl isophthalate
- MBTP:
-
mono-n-butyl terephthalate
- PA:
-
phthalic acid
- IPA:
-
isophthalic acid
- TPA:
-
terephthalic acid
- PCMB:
-
p-chloromercuribenzoate
- BSA:
-
bovine serum albumin
References
Carter BR, Cook MW, Gangolli SD, Grasso P (1977) Studies on dibutyl phthalate induced testicular atrophy in the rat. Effect on zinc metabolism. Toxicol Appl Pharmacol 41: 609–618
Foster PMD, Lake BG, Thomas LV, Cook MW, Gangolli SD (1982) Studies on the testicular effects and zinc excretion produced by various isomers of monobutyl-o-phthalate in the rat. Chem-Biol Interact 34: 233–238
Gangolli SD (1982) Testicular effects of phthalate esters. Environ Health Perspect 45: 77–84
Gray TJB, Rowland IR, Foster PMD, Gangolli SD (1982) Species differences in the testicular toxicity of phthalate esters. Toxicol Lett 11: 141–147
Ikemoto I, Kodera S, Katsurai K, Inaba Y, Machida T, Tanaka A (1983) Experimental testicular damage induced by dibutyl phthalate and monobutyl phthalate. Jap Fert Ster 28: 160–165
Lake BG, Phillips JC, Linnell JC, Gangolli SD (1977) The in vitro hydrolysis of some phthalate diesters by hepatic and intestinal preparations from various species. Toxicol Appl Pharmacol 39: 239–248
Levy M, Ocken PR (1969) Purification and properties of pig liver esterase. Arch Biochem Biophy 135: 259–264
Oishi S, Hiraga K (1980) Testicular atrophy induced by phthalic acid esters: effect on testosterone and Zn concentration. Toxicol Appl Pharmacol 53: 35–41
Takahashi T, Tanaka A, Yamaha T (1981) Elimination, distribution and metabolism of 2-(ethylhexyl) adipate (DEHA) in rats. Toxicology 22: 223–233
Tanaka A, Matsumoto A, Yamaha T (1978) Biochemical studies on phthalic esters. III. Metabolism of dibutyl phthalate (DBP) in animals. Toxicology 9: 109–123
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takahashi, T., Tanaka, A. Biochemical studies on phthalic esters V. Arch Toxicol 63, 72–74 (1989). https://doi.org/10.1007/BF00334638
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00334638