Summary
The effects of distant deletions or insertions in the Salmonella typhimurium donor strains on P22-mediated cotransducibility of genetic markers was studied. We found that deletions of histidine operon, unit 44 of the chromosome map, changed the linkage of markers purF and aroC (unit 49) and pyrF and trpA (unit 34). They did not change the linkage of more distant markers pyrE and cysE. The effect of three types of insertions was examined. The donor strains carried F factor, Tn10 transposon or pi-his duplication inserted close to histidine operon. These insertions caused alteration of purF-aroC linkage while pyrF-trpA cotransduction values were not affected. These data show that the effect of the chromosome rearrangements extends to at least 5% of S. typhimurium chromosome length and may reach as much as 10% of it. Our results are in agreement with the model of Chelala and Margolin (1974) concerning formation of transduction particles. They indicate that the cotransducibility changes caused by deletions or insertions extent further than it might have been expected from previous reports.
Similar content being viewed by others
References
Anderson, R.P., Roth, J.R.: Tandem genetic duplications in phage and bacteria. Ann. Rev. Microb. 31, 473–505 (1977)
Anderson, R.P., Roth, J.R.: Tandem chromosomal duplications in Salmonella typhimurium: Fusion of histidine genes to novel promoters. J. molec. Biol. 119, 147–166 (1978)
Chelala, C.A., Margolin, P.: Effect of deletions on cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletions affect the formation of transducing DNA fragments. Mol. Gen. Genet. 131, 97–112 (1974)
Chelala, C.A., Margolin, P.: Evidence that HT mutant strains of bacteriophage P22 retain an altered form of substrate specificity in formation of transducing particles in Salmonella typhimurium. Genet. Res. (Camb.) 27, 315–322 (1976)
Jackson, E.N., Jackson, D.A., Deans, R.J.: EcoRI analysis of bacteriophage P22 DNA packaging. J. molec. Biol. 118, 365–388 (1978)
Kemper, J.: Gene order and cotransduction in the leu-ara-fol-pyrA region of the Salmonella typhimurium linkage map. J. Bact. 117, 94–99 (1974)
Kleckner, N., Roth, J., Botstein, D.: Genetic engineering in vivo using translocable drug resistance elements. New methods in bacterial genetics. J. molec. Biol. 117, 125–159 (1977)
Murray, M.L., Kłopotowski, T.: Genetic map position of the gluconate-6-phosphate dehydrogenase gene in Salmonella typhimurium. J. Bact. 95, 1279–1282 (1968)
Ohtsubo, H., Ohtsubo, E.: Repeated DNA sequences in plasmids, phages and bacterial chromosomes. In: DNA insertion elements, plasmids and episomes (Boukhari, A.I., Shapiro, J.A., and Adhya, S.L., eds.) ppl. 49–63. New York: Cold Spring Harbor Laboratory 1977
Sanderson, K.E., Hartman, P.E.: Linkage map of Salmonella typhimurium. Edition V. Microbiol. Revs. 42, 471–519 (1978)
Sanderson, K.E., Ross, H., Ziegler, L., Mäkelä, P.H.: F+, Hfr, and F′ strains of Salmonella typhimurium and Salmonella abony. Bact. Rev. 36, 608–637 (1972)
Schmieger, H.: Phage P22-mutants with increased or decreased transduction abilities. Mol. Gen. Genet. 119, 75–88 (1972)
Schmieger, H., Backhaus, H.: Altered cotransduction fraquencies exhibited by HT-mutant of Salmonella-phage P22. Mol. Gen. Genet. 143, 307–309 (1976)
Smith, H.O., Levine, M.: A phage gene controlling integration of prophage. Virology 31, 207–216 (1967)
Vogel, H.H., Bonner, D.M.: Acetylornithase of Escherichia coli: Partial purification and some properties. J. biol. Chem. 218, 97–106 (1956)
Wu, T.T.: A model for three-point analysis of random general transduction. Genetics 54, 405–410 (1966)
Author information
Authors and Affiliations
Additional information
Communicated by G. O'Donovan
Rights and permissions
About this article
Cite this article
Krajewska-Grynkiewicz, K., Kłopotowski, T. Altered linkage values in phage P22-mediated transduction caused by distant deletions or insertions in donor chromosomes. Molec. Gen. Genet. 176, 87–93 (1979). https://doi.org/10.1007/BF00334299
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334299