Summary
With the help of recombinant plasmids carrying the recA gene of Escherichia coli or of Proteus mirabilis the ability of the recA gene products to substitute functionally for each other was studied. The recA protein of each can function in recombination, repair, induction of mutations and prophages and in regulation of its own synthesis within the foreign host nearly equally well as in the natural host. It is, therefore, suggested that recA-dependent processes act similarly in E. coli and P. mirabilis.
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References
Bagg A, Kenyon CJ, Walker GC (1981) Indicibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci USA 78:5749–5753
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening of recombinant plasmid DNA. Nucl Acids Res 7:1513–1523
Böhme H (1963) Transduction of prototrophy to auxotrophic mutants of Proteus mirabilis. Biochem Biophys Res Commun 10:60–62
Böhme H (1968) Absence of repair of photodynamic induced damage in two mutants of Proteus mirabilis with increased sensitivity to monofunctional alkylating agents. Mutat Res 6:166–168
Bresler SE, Krivonogov SV, Lanzov VA (1978) Scale of the genetic map and genetic control of recombination after conjugation in Escherichia coli K-12. Hot spots of recombination. Mol Gen Genet 166:337–346
Cassuto E, West SC, Mursalim J, Conlon S, Howard-Flanders P (1980) Initiation of genetic recombination: Homologous pairing between duplex DNA molecules promoted by recA protein. Proc Natl Acad Sci USA 77:3962–3966
Clarke L, Carbon J (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire Escherichia coli genome. Cell 9:91–99
Eitner G, Adler B, Frank B (1980) High-level synthesis of the reA protein is not sufficient for the expression of SOS-functions in Escherichia coli K-12. Biol Zbl 99:443–451
Eitner G, Solonin AS, Tanyashin VI (1981) Cloning of a recA-like gene of Proteus mirabilis. Gene 14:301–308
Emmerson PT, West SC (1977) Identification of protein X of Escherichia coli as the recA +/tif+gene product. Mol Gen Genet 155:77–85
Gudas LJ (1976) The induction of protein X in DNA repair and cell division mutants of E. coli. J Mol Biol 104:567–587
Gudas LJ, Mount DW (1977) Identification of the recA(tif) gene product of Escherichia coli. Proc Natl Acad Sci USA 74:5280–5284
Hall JD, Mount DW (1981) Mechanisms of DNA replication and mutagenesis in ultraviolet-irradiated bacteria and mammalian cells. In: Cohn WD (ed) Progress in nucleic acid research and molecular biology, vol 25. Academic Press, New York, p 54
Hofemeister J (1977) DNA repair in Proteus mirabilis IV. Post-irradiation DNA degradation as influenced by a function inducible in rec+ cells. Mol Gen Genet 154:35–41
Hofemeister J, Böhme H (1975) DNA repair in Proteus mirabilis III. Survival, dimer excision, and UV reactivation in comparison with Escherichia coli K-12. Mol Gen Genet 141:147–161
Hofemeister J, Eitner G (1981) Repair and plasmid R46 mediated mutation requires inducible functions in Proteus mirabilis. Mol Gen Genet 183:369–375
Hofemeister J, Fleischhacker M, Adler B, Eitner G, Steinborn G, Böhme H (1979a) DNA repair in Proteus mirabilis V. Post-irradiation response and the defective lysogeny of strains derived from PGVI. Biol Zbl 98:315–332
Hofemeister J, Köhler H, Filippov VD (1979b) DNA repair in Proteus mirabilis VI. Plasmid(R46-) mediated recovery and UV mutagenesis. Mol Gen Genet 76:265–273
Horii T, Ogawa T, Ogawa H (1980) Organization of the recA gene of Escherichia coli. Proc Natl Acad Sci USA 77:313–317
Howard-Flanders P, Thériot L (1966) Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53:1137–1150
Kato T, Shinoura Y (1977) Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by UV light. Mol Gen Genet 156:121–131
Kenyon CJ, Walker GC (1980) DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci USA 77:2819–2823
Kooistra J, Setlow JK (1976) Similarity in properties and mapping of three rec mutants of Haemophilus influenzae. J Bacteriol 127:327–333
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Law S, Tamoaki T, Kreuzaler F, Dugaicyk A (1980) Molecular cloning of DNA complementary to a mouse α-fetoprotein mRNA sequence. Gene 10:53–61
Little JW, Edmiston SH, Pacelli LZ, Mount D (1980) Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci USA 77:3225–3229
Mac Phee DG (1970) Recombination-deficient mutants of colicinogenic Salmonella typhimirium detected by their failure to produce colicin. J Bacteriol 104:345–350
McEntee K (1977) Protein X is the product of the recA gene of Escherichia coli. Proc Natl Acad Sci USA 74:5275–5279
Radman M (1974) Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. In: Miller M (ed) Molecular and environmental aspects of mutagenesis. C.C. Thomas, Springfield III., p 128–142
Roberts WJ, Roberts CW, Craig NC (1978) Escherichia coli recA gene product inactivates phage lambda repressor. Proc Natl Acad Sci USA 75:4714–4718
Sancar A, Rupp WD (1979) Physical map of the recA gene. Proc Natl Acad Sci USA 76:3144–3148
Sancar A, Stachelek C, Konigsberg W, Rupp WD (1980) Sequences of the recA gene and protein. Proc Natl Acad Sci USA 77:2611–2615
Sedgwick SG, Levine A, Bailone A (1978) Induction of recA +-protein synthesis in Escherichia coli. Mol Gen Genet 160:267–276
Shibata T, Dasgupta C, Cunningham RP, Radding CM (1980) Homologous pairing in genetic recombination: Formation of D loops by combined action of recA protein and a helix destabilizing protein. Proc Natl Acad Sci USA 77:2606–2610
Steinborn G (1978) Uvm Mutants of Escherichia coli K-12 deficient in UV mutagenesis I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis. Mol Gen Genet 165:87–93
Witkin EW (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907
Weinstock GM, McEntee K, Lehman IR (1979) ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli. Proc Natl Acad Sci USA 76:126–131
Yagi Y, Clewell DP (1980) Recombination-deficient mutant of Streptococcus faecalis. J Bacteriol 143:966–970
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Communicated by B.A. Bridges
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Eitner, G., Adler, B., Lanzov, V.A. et al. Interspecies recA protein substitution in Escherichia coli and Proteus mirabilis . Mol Gen Genet 185, 481–486 (1982). https://doi.org/10.1007/BF00334144
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DOI: https://doi.org/10.1007/BF00334144