Skip to main content
Log in

Transcriptional organization of the rpsA operon of Escherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Three strong and two minor rpsA promoters were found by nuclease S1 mapping, promoter cloning and in vitro transcription. The longest transcript encodes a protein, located upstream from rpsA with a molecular weight of 25,000. The identity of this protein remains to be established. The other rpsA promoters are located within the gene for this 25 K protein. The rpsA leader region including the sequence of the 25K protein and its promoter was DNA sequenced.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aiba H, Adhya S, deCrombrugghe B (1981) Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem 256:11905–11910

    Google Scholar 

  • Bachmann B (1983) Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev 47:180–230

    Google Scholar 

  • Berk JA, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732

    Google Scholar 

  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113

    Google Scholar 

  • Brown S, Albrechtsen B, Pedersen S, Klemm P (1982) Localization and regulation of the structural gene for transcription termination factor rho of Escherichia coli. J Mol Biol 162:283–298

    Google Scholar 

  • Casadaban MJ, Cohen SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179–207

    Google Scholar 

  • Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bact 134:1141–1156

    Google Scholar 

  • Christiansen L, Pedersen S (1981) Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1. Mol Gen Genet 181:548–551

    Google Scholar 

  • Fukuda R, Iwakura Y, Ishihama A (1974) Heterogeneity of RNA polymerase in Escherichia coli. I. A new holoenzyme containing a new sigma factor. J Mol Biol 83:353–367

    Google Scholar 

  • Gonzalez N, Wiggs J, Chamberlin M (1977) A simple procedure for resolution of Escherichia coli RNA polymerase holoenzmye from core polymerase. Arch Biochem Biophys 182:404–408

    Google Scholar 

  • Hansen FG, Hansen EB, Atlung T (1982) The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for ribosomal protein L34, of Escherichia coli. Embo J 1:1043–1046

    Google Scholar 

  • Hawley D (1983) Compilation and analysis of E. coli promoter DNA sequences. Nucl Acids Res 11:2237–2255

    Google Scholar 

  • Kajitani M, Ishihama A (1983a) Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli. Nucl Acids Res 11:671–686

    Google Scholar 

  • Kajitani M, Ishihama A (1983b) Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from E. coli. Nucl Acids Res 11:3873–3888

    Google Scholar 

  • Kajitani M, Ishihama A (1984) Promoter selectivity of Escherichia coli RNA polymerase, I. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons. J Biol Chem, in press

  • Kitikawa M, Blumenthal L, Isono K (1980) Isolation and characterization of specialized transducing lambda phages carrying ribosomal protein genes of E. coli. Mol Gen Genet 180:343–349

    Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–566

    Google Scholar 

  • Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucl Acids Res 9:309–321

    Google Scholar 

  • Ono M, Kuwano M (1979) Genetic analysis of a mutation affecting ribosomal protein S1 in E. coli. Mol Gen Genet 174:11–15

    Google Scholar 

  • Pedersen S, Reeh S, Friesen JD (1978) Functional mRNA half lives in E. coli. Mol Gen Genet 166:329–336

    Google Scholar 

  • Pedersen S, Skouv J, Christensen T, Johnsen M, Fiil N (1982) Ribosomal protein L10 and S1 control their own synthesis. In: Grunberg-Manago M, Safer B (eds) Interaction of translational and transcriptional controls in the regulation of gene expression. Elsevier, New York, p 119

    Google Scholar 

  • Reeh S, Pedersen S, Friesen JD (1976) Biosynthesis regulation of individual proteins in relA + and relA strains of Escherichia coli during amino acid starvation. Mol Gen Genet 149:279–289

    Google Scholar 

  • Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693

    Google Scholar 

  • Schnier J, Isono K (1982) The DNA sequence of the gene rpsA of E. coli coding for ribosomal protein S1. Nucl Acids Res 10:1858–1865

    Google Scholar 

  • Steitz J (1979) Genetic signals and nucleotide sequences in messenger RNA. In: Goldberger RF (ed) Biological regulation and development, vol 1. Plenum Press, New York, p 349

    Google Scholar 

  • Subramanian A-R (1983) Structure and functions of ribosomal protein S1. Progr Nucl Acid Res Molec Biol 28:102–138

    Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by K. Isono

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pedersen, S., Skouv, J., Kajitani, M. et al. Transcriptional organization of the rpsA operon of Escherichia coli . Mol Gen Genet 196, 135–140 (1984). https://doi.org/10.1007/BF00334105

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334105

Keywords

Navigation