Summary
Haploid and diploid wild types and UV-sensitive (uvs 1–3) strains were exposed to UV light in stationnary phase of growth and in log phase. The liquid hold recovery (LHR) was studied in both conditions. 1. It appears that haploid wild type resting cells (1st type of repair) are less capable of repair during dark holding than dividing cells (2nd type of repair). 2. The mutant uvs 1–3, which behaves like an excision defective strain, has lost the 1st type of repair. In contrast, the 2nd type of repair is still present. 3. The LHR is not additive to photorestoration (PR) for the 1st type of repair. On the contrary LHR and PR are additive for the 2nd type of repair. 4. Caffeine suppresses the 1st type of recovery and has only a slight effect on the 2nd type. 5. Both types of repair are functionning in diploid wild type cells where only the 2nd type of repair is present in UV-sensitive homozygous diploids uvs 1/uvs 1.
From these data it is tentatively suggested that the 1st type of repair is related to the excision-resynthesis repair mechanism. The 2nd type of repair, active in dividing haploid cells and in diploid cells, may involve chromosomal exchanges.
The effect of storage in the dark for the cytoplasmic “petite” induction by UV was examined in wild type strains. A negative liquid holding (NLH) effect (increase of the frequency of “petites” during storage) was observed for diploid cells and after low doses (up to 1,500 ergs/mm2) for haploid cells. At high doses a recovery is observed in haploid cells. An interpretation of this NLH effect is discussed. This differential response to dark holding for the lethal damage and cytoplasmic genetic damage supports the idea that there is a certain degree of independence between the nuclear and the mitochondrial systems with regard to the repair machinery.
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Moustacchi, E., Enteric, S. Differential “liquid holding recovery” for the lethal effect and cytoplasmic “petite” induction by UV light in Saccharomyces cerevisiae . Molec. Gen. Genetics 109, 69–83 (1970). https://doi.org/10.1007/BF00334047
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DOI: https://doi.org/10.1007/BF00334047