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Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae

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Summary

Ribosomal proteins from the cytoplasm and mitochondria of the yeast Saccharomyces cerevisiae were compared by immunoblotting techniques. Antibodies raised against cytoplasmic ribosomal proteins cross-react with five mitochondrial ribosomal proteins, four of which are located in the large and one in the small mitochondrial subunits. The possible existence of common ribosomal proteins for cytoplasmic and mitochondrial ribosomes is discussed.

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Abbreviations

cyto:

cytoplasmic

mito:

mitochondrial

References

  • Avadhani NG, Lewis FS, Rutman RJ (1975) Mitochondrial RNA and protein metabolism. Subcell Biochem 4:93–145

    Google Scholar 

  • Barritault D, Expert-Bezançon A, Guerin MF, Hayes D (1976) The use of acetone precipitation in the isolation of ribosomal proteins. Eur J Biochem 63:131–135

    Google Scholar 

  • Boynton LE, Gillhan NW, Lambowitz AM (1980) Biogenesis of chloroplast and mitochondrial ribosomes. In: Chambliss G, Graven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes: structure, function and genetics. Univ Park Press, Baltimore, pp 903–950

    Google Scholar 

  • Breining P, Surguchov AP, Piepersberg W (1984) Cloning and identification of a dna fragment for the sup 1 gene of Saccharomyces cerevisiae. Current Genet 8:467–470

    Google Scholar 

  • Buetow DE, Wood WM (1978) The mitochondrial translation system. Subcell Biochem 5:1–85

    Google Scholar 

  • Burnette WN (1981) “Western Blotting”: electrophoretic transfer of proteins from SDS-polyacrylamide gels to unmodified nitrocellulose and radioactive detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203

    Google Scholar 

  • Chua NH, Bennoun P (1975) Site of synthesis of thylakoid membrane proteins. Proc Natl Acad Sci USA 72:2175–2179

    Google Scholar 

  • Faye G, Sor F (1977) Analysis of mitochondrial ribosomal proteins of Saccharomyces cerevisiae by two dimensional polyacrylamide gel electrophoresis. Mol Gen Genet 55:27–34

    Google Scholar 

  • Gabius HJ, Engelhardt R, Piel N, Stermbach H, Cramer F (1982) Phenylalanyl-tRNA synthetases from yeast cytoplasm and mitochondria. Biochim Biophys Acta 743:451–454

    Google Scholar 

  • Grivell LA, Netter P, Borst P, Slonimski PP (1973) Ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin. Biochim Biophys Acta 312:358–367

    Google Scholar 

  • Hallermeyer G, Neupert W (1975) Immunological differences of mitochondrial and cytoplasmic ribosomes of Neurospora crassa. FEBS Lett 41:264–268

    Google Scholar 

  • Hooper AK, Furukawa AM, Pham HD, Martin NC (1982) Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutations. Cell 28:543–550

    Google Scholar 

  • Ichiguro J, Arakatsu J (1975) Comparative electrophoretic study on proteins from mitochondrial and cytoplasmic ribosomes of Saccharomyces cerevisiae. Plant Cell Physiol 16:753–758

    Google Scholar 

  • Kuntzel H (1969) Proteins of mitochondrial and cytoplasmic ribosomes of Neurospora crassa. Nature 222:142–146

    Google Scholar 

  • Leister DE, David JB (1974) Physical properties and protein constituents of cytoplasmic and mitochondrial ribosomes of Xenopus laevis. J Biol Chem 249:5108–5118

    Google Scholar 

  • Martin NC, Hopper AK (1982) Isopentilation of both cytoplasmic and mitochondrial tRNAs is affected by a single nuclear mutation. J Biol Chem 257:10562–10565

    Google Scholar 

  • Metz L, Bogorad L (1974) Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins. Anal Biochem 57:200–210

    Google Scholar 

  • Morimoto H, Halvorson HO (1971) Characterization of mitochondrial ribosomes from yeast. Proc Natl Acad Sci USA 68:324–328

    Google Scholar 

  • Neupert W (1977) Mitochondrial ribosomes. In: Quagliarello E et al. (eds) Horyzons in biochemistry and biophysics, vol 3. Addison-Wesley Publ Comp Inc, London, pp 257–296

    Google Scholar 

  • O'Brien TW, Mattews DE (1976) Mitochondrial ribosomes. In: King RC (ed) Handbook of genetics. Plenum Press, New York, pp 535–580

    Google Scholar 

  • Paley EL, Baranov VN, Zinoviev NE, Kisselev LL (1985) Immunomorphological and biochemical studies on intracellular distribution of tryptophanyl-tRNA synthetase from higher eukariotes. Biopolim Cell (in Russian) 1:70–75

    Google Scholar 

  • Peacock AC, Dingman CW (1968) Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674

    Google Scholar 

  • Schmitt H (1971) Core particles and proteins from mitochondrial ribosomes of yeast. FEBS Lett 16:186–190

    Google Scholar 

  • Schwartz K, Lompre AM, Bouveret P, Wisnevsky C, Whalen RG (1982) Comparison of rat cardiac myosins at fetal stages in young animals and in hypothyroid adults. J Biol Chem 257:14412–14418

    Google Scholar 

  • Surguchov AP, Sudarickov AB, Telckov MV, Smirnov VN, Ter-Avanesyan MD, Inge-Vechtomov SG (1983) Relationship between cytoplasmic and mitochondrial apparatus of protein synthesis in yeast Saccharomyces cerevisiae. Mol Gen Genet 189:172–174

    Google Scholar 

  • Surguchov AP, Smirnov VN, Ter-Avanesyan MD, Inge-Vechtomov SG (1984) Ribosomal suppressors in eukaryots In: Skulachev VP (ed) Soviet scientific reviews, vol 4. Harwood Acad Publishers, New York, pp 147–205

    Google Scholar 

  • Surguchov AP, Fominykh ES, Tonevitsky AG, Telckov MV (1985) Identification of the sup1 gen product. In: Sosinov AA (eds) Molecularnye mechanismy geneticheskikh processor. Moscow Nauka pp 315–323

    Google Scholar 

  • Ter-Avanesyan MD, Zimmermann J, Inge-Vechtomov SG, Sudarickov AB, Smirnov VN, Surguchov AP (1982) Ribosomal recessive suppressors cause a respiratory deficiency in yeast Saccharomyces cerevisiae. Mol Gen Genet 185:319–323

    Google Scholar 

  • Van der Zeijst Ben AM, Kool AJ, Bloemers HPJ (1972) Isolation of active ribosomal subunits from yeast. Eur J Biochem 30:15–25

    Google Scholar 

  • Vasconcelos ACL, Bogorad L (1971) Proteins of cytoplasmic, chloroplast and mitochondrial ribosomes of some plants. Biochim Biophys Acta 228:492–502

    Google Scholar 

  • Wood WG, Wachter C, Scriba PC (1981) Experiences using Chloramine-T and Iodogen for radioiodination of materials for immunoassay. J Clin Chem Clin Biochem 19:1051–1058

    Google Scholar 

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Communicated by D. Goldfarb

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Sudarickov, A.B., Surguchov, A.P. Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae . Molec Gen Genet 203, 316–319 (1986). https://doi.org/10.1007/BF00333973

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  • DOI: https://doi.org/10.1007/BF00333973

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