Summary
A screening method, based upon resistance to a tetrazolium salt (TTC), is described which permitted the isolation in Paramecium of 28 mutants resistant to TTC. These mutants displayed various defects in mitochondrial functions (cytochromic content, cyanide insensitive respiration). Some mutations seemed to affect directly the respiration chain while others seemed to cause indirect modifications, possibly altering mitochondrial protein synthesis.
Genetic analysis of four mutants showed in all that the resistance to TTC was of nuclear origin.
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Adoutte, A., Doussière, J.: Physiological consequences of mitochondrial antibiotic-resistant mutations in Paramecium: Growthrates, cytochromic defects and cyanide-insensitive respiration of mutant and erythromycin-treated wild-type strains. Molec. gen. Genet. 1978 (In press)
Bertrand, H., Nargang, F.E., Collins, R.A., Zagogeski, C.A.: Nuclear cytochrome-deficient mutants of Neurospora crassa: isolation, characterization, and genetic mapping. Molec. gen. Genet. 153, 247–257 (1977)
Claisse, M., Péré-Aubert, G.A., Clavillier, L.P., Slonimski, P.P.: Méthode d'estimation de la concentration des cytochromes dans les cellules entières de levure. Europ. J. Biochem. 16, 430–438 (1970)
Doussière, J., Adoutte, A., Sainsard, A., Ruiz, F., Beisson, J., Vignais, P.V.: Genetical and physiological analysis of the respiratory chain of Paramecium. In: Genetics and Biogenesis of Chloroplasts and Mitochondria (Th. Bücher, W. Neupert, W. Sebald and S. Werner, ed.), pp. 873–880. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Ebner, E., Mason, T.L., Schatz, G.: Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. II. Effets of nuclear and extrachromosomal mutations on the formation of cytochrome c oxidase. J. biol. Chem. 248, 5369–5378 (1973b)
Ebner, E., Menucci, L., Schatz, G.: Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. I. Effets of nuclear mutations on mitochondrial protein synthesis. J. biol. Chem. 248, 5360–5368 (1973a)
Edwards, D.L., Kwiecinski, F., Horstmann, J.: Selection of respiratory mutants of Neurospora crassa. J. Bact. 114, 164–168 (1973)
Edwards, D.L., Rosenberg, E., Maroney, P.A.: Induction of cyanide-insensitive respiration in Neurospora crassa. J. biol. Chem. 249, 3551–3556 (1974)
Gillie, O.J.: Methods for the study of nuclear and cytoplasmic variation in respiratory activity of Neurospora crassa, and the discovery of three new genes. J. gen. Microbiol. 61, 379–395 (1970)
Goffeau, A., Colson, A.M., Landry, Y., Foury, F.: Modifications of mitochondrial ATPase in chromosomal respiratory-deficient mutants of a “petite negative” yeast: Schizosaccharomyces pombe 972h-. Biochem. biophys. Res. Commun. 48, 1448–1454 (1972)
Goffeau, A., Labaille, F., Mohar, O., Colson, A.M.: Screening tests for suppressors of respiratory deficient mutants in Schizosaccharomyces pombe and model for a mitochondrial partial suppression of a nuclear pleiotropic strain (Th. Bücher, W. Neupert, W. Sebald and S. Werner, ed.), pp. 851–856. Amsterdam: Elsevier/North-Holland Biomedical Press 1976
Henry, M.F., Nyns, E.J.: Cyanide-insensitive respiration. An alternative mitochondrial pathway. Sub. Cell. Biochem. 4, 1–65 (1975)
Klein, J.L., Edwards, D.L.: Regulation of mitochondrial membrane assembly in Neurospora crassa. J. biol. Chem. 250, 5852–5858 (1975)
Ogur, M., St John, R., Nagai, S.: Tetrazolium overlay technique for population studies of respiratory deficiency in Yeast. Science 125, 928–929 (1957)
Sainsard A., Claisse, M., Balmefrézol, M.: A nuclear mutation affecting structure and function of mitochondria in Paramecium. Molec. gen. Genet. 130, 113–125 (1974)
Sainsard-Chanet, A.: A new type of mitochondrial mutation in Paramecium. Molec. gen. Genet. 159, 117–123 (1978)
Schatz, G., Mason, T.L.: The biosynthesis of mitochondrial proteins. Ann. Rev. Biochem. 43, 51–87 (1974)
Schonbaum, G.S., Bonner, W.D. Jr., Storey, B.T., Bahr, J.T.: Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 47, 124–128 (1971)
Slater, T.F., Sawyer, B., Sträuli, U.: Studies on succinate-tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts. Biochim. biophys. Acta (Amst.) 77, 383–393 (1963)
Sonneborn, T.M.: Methods in Paramecium research. In: Methods in cell, physiology (D. Prescott, ed.), Vol. 4, pp. 241–339. Oxford: Academic Press 1970
Sonneborn, T.M.: The Paramecium aurelia complex of fourteen sibling species. Trans. Amer. mier. Soc. 94, 155–178 (1975)
Tait, A.: Altered mitochondrial ribosomes in a erythromycin resistant mutant of Paramecium. FEBS Lett. 24, 117–120 (1972)
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Ruiz, F., Adoutte, A. Selection and characterization of nuclear mutations affecting mitochondria in Paramecium . Molec. Gen. Genet. 162, 1–8 (1978). https://doi.org/10.1007/BF00333844
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DOI: https://doi.org/10.1007/BF00333844