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The localization of tRNA Asp2 genes from Drosophila melanogaster by “in situ” hybridization

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Summary

Transfer RNA Asp2δ was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated “in vitro” with Na [125I] and hybridized “in situ” to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNA Asp2 to the left arm of the second chromosome in the regions 29 D and E.

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Authors and Affiliations

  1. Zoologisch-Vergl. Anatomisches Institut der Universität Zürich, Künstlergasse 16, CH-8006, Zürich, Switzerland

    Thomas Schmidt, Albert H. Egg & Eric Kubli

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  1. Thomas Schmidt
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  2. Albert H. Egg
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  3. Eric Kubli
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Communicated by W. Gehring

This work was supported by grant No. 3.024.76 from the Swiss National Science Foundation and the Hescheler-Stiftung. We wish to express our gratitude to Drs. St. Clarkson and D. Söll for reading the manuscript

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Schmidt, T., Egg, A.H. & Kubli, E. The localization of tRNA Asp2 genes from Drosophila melanogaster by “in situ” hybridization. Molec. Gen. Genet. 164, 249–254 (1978). https://doi.org/10.1007/BF00333153

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  • DOI: https://doi.org/10.1007/BF00333153

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