Summary
Plasmid inter-relationships were studied by hybridisation of a radioactively labelled DNA probe to endonuclease-derived fragmentation patterns of plasmids bound to a nitrocellulose filter. The degradative plasmids SAL and NAH were found to be very closely related, but probably one did not give rise to the other by just a single deletion or insertion. Relationships between SAL and other degradative plasmids are complex; substantial homology was found with TOL and other plasmids encoding toluate dissimilation and significant homology was found with OCT.
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Communicated by Ch. Auerbach
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Heinaru, A.L., Duggleby, C.J. & Broda, P. Molecular relationships of degradative plasmids determined by in situ hybridisation of their endonuclease-generated fragments. Molec. Gen. Genet. 160, 347–351 (1978). https://doi.org/10.1007/BF00332979
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DOI: https://doi.org/10.1007/BF00332979