Summary
P1 DNA is synthesized in the E. coli ts dna mutants 165/70 (elongation defect) and 252 (initiation defect) at elevated temperatures. In strain 165/70, P1 infection at 41°C leads to phage production accompanied by a transient recovery of bacterial DNA synthesis. No phages are produced byt P1 DNA is still synthesized in strain 252 if infected after host DNA replication has come to a halt at 42°C.
Similar content being viewed by others
References
Beyersmann, D., Schlicht, M., Schuster, H.: Temperature-sensitive initiation of DNA replication in a mutant of Escherichia coli K12. Molec. gen. Genet. 111, 145–158 (1971).
Denhardt, D. T.: A membrane-filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun. 23, 641–646 (1966).
Lanka, E., Schuster, H.: Replication of bacteriophages in Escherichia coli mutants thermosensitive in DNA synthesis. Molec. gen. Genet. 106, 274–285 (1970).
Mikolajczyk, M., Schuster, H.: The fate of newly made DNA in Escherichia coli mutants thermosensitive in DNA synthesis. Molec. gen. Genet. 110, 272–288 (1971).
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Beyersmann, D., Schuster, H. DNA synthesis in P1 infected E. coli mutants temperature-sensitive in DNA replication. Molec. Gen. Genetics 114, 173–176 (1972). https://doi.org/10.1007/BF00332788
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00332788