Summary
DNA was isolated from chloroplasts of Euglena gracilis var. bacillaris (ATCC No. 10616). The structure of the rDNA was studied using partial denaturation mapping and heteroduplex analysis. Seven GC-rich stretches representing the rDNA were apparent in the partial denaturation pattern. To analyse the structure of the rDNA in detail, heteroduplexes with the E. coli rrnD operon cloned in the plasmid pBK8 were prepared. Five complete rRNA operons were found. In addition one extra 16S rRNA gene was located between the second and third complete operons and another extra 16S rRNA gene was upstream of all five operons. Each one was associated with a small inverted repeat structure.
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Communicated by J. Schell
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Koller, B., Delius, H. A chloroplast DNA of Euglena gracilis with five complete rRNA operons and two extra 16S rRNA genes. Mol Gen Genet 188, 305–308 (1982). https://doi.org/10.1007/BF00332692
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DOI: https://doi.org/10.1007/BF00332692