Summary
R. leguminosarum trp alleles mapped by R68.45-mediated recombination were located in three distinct chromosomal regions. We isolated three derivatives of R68.45 that carried different trp genes of R. meliloti. Each of the plasmids suppressed all of the R. leguminosarum trp alleles in a particular region. The R-primes were transferred to strains of P. aeruginosa carrying mutations in different trp genes. The plasmid pAJ24JI suppressed trpA, B and F mutants, pAJ73JI suppressed trpC and D and pAJ88JI suppressed a trpE mutant. When the R-primes were transferred to E. coli trp strains they failed to suppress any trp mutants. A derivative of pAJ24JI was isolated which was able to suppress trpA and F mutants of E. coli.
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Beringer, J.E.: R-factor transfer in Rhizobium leguminosarum. J. gen. Microbiol. 84, 188–198 (1974)
Beringer, J.E., Hoggan, S.A., Johnston, A.W.B.: Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. J. gen. Microbiol. 104, 201–207 (1978)
Beringer, J.E., Hopwood, D.A.: Chromosomal mobilization and mapping in Rhizobium leguminosarum. Nature (Lond.) 264, 291–293 (1976)
Bibb, M.J., Freeman, R.F., Hopwood, D.A.: Physical and genetical characterization of a second sex factor SCP2, for Streptomyces coelicolor A3(2). Molec. gen. Genet. 154, 155–166 (1977)
Bishop, P.E., Dazzo, F.B., Appelbaum, E.R., Maier, R.J., Brill, W.J.: Intergeneric transfer of genes involved in the Rhizobium-legume symbiosis. Science 198, 938–940 (1976)
Cannon, F.C., Postgate, J.R.: Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter. Nature (Lond.) 260, 271–272 (1976)
Crawford, I.P.: Gene rearrangements in the evolution of the tryptophan pathway. Bact. Rev. 39, 87–120 (1975)
Dixon, R., Cannon, F., Kondorosi, A.: Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature (Lond.) 260, 268–271 (1976)
Dunican, L.K., Tierney, A.B.: Genetic transfer of nitrogen fixation from Rhizobium trifolii to Klebsiella pneumoniae. Biochem. Biophys. Res. Commun. 57, 62–72 (1974)
Grinsted, J., Bennett, P.M., Richmond, M.H.: A restriction enzyme map of R-plasmid RP1. Plasmid 1, 34–37 (1977)
Haas, D., Holloway, B.W.: R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molec. gen. Genet. 144, 243–257 (1976)
Hedges, R.W., Jacob, A.E., Crawford, I.P.: Wide ranging plasmid bearing the Pseudomonas aeruginosa tryptophan synthase genes. Nature (Lond.) 267, 283–284 (1977)
Hopwood, D.A., Wright, H.M.: A plasmid of Streptomyces coelicolor carrying a chromosomal locus and its interspecific transfer. J. gen. Microbiol. 79, 331–342 (1973)
Hu, S., Ohtsubo, E., Davison, N.: Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. J. Bact. 122, 749–763 (1974)
Jacob, A.E., Cresswell, J.M., Hedges, R.W.: Molecular characterisation of the P group plasmid R68 and variants with enhanced chromosome mobilizing ability. FEMS Lett. 1, 71–74 (1977)
Jacob, A.E., Cresswell, J.M., Hedges, R.W., Coetzee, J.N., Beringer, J.E.: Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum and Proteus mirabilis into RP4. Molec. gen. Genet. 147, 315–323 (1976)
Jacob, F., Wollman, E.L.: In: Sexuality and the genetics of bacteria. New York: Academic Press 1961
Johnston, A.W.B., Beringer, J.E.: Identification of the Rhizobium strains in pea root nodules using genetic markers. J. gen. Microbiol. 87, 343–350 (1975)
Johnston, A.W.B., Setchell, S.M., Beringer, J.E.: Interspecific crosses between Rhizobium leguminosarum and R. meliloti: formation of haploid recombinants and of R-primes. J. gen. Microbiol. 104, 209–218 (1978)
Kondorosi, A., Kiss, G.B., Forrai, T., Vincze, E. Banfalvi, Z.: Circular linkage map of the Rhizobium meliloti chromosome. Nature (Lond.) 268, 525–527 (1977)
Nagahari, K., Sano, Y., Sakaguchi, K.: Derepression of E. coli trp operon on interfamilial transfer. Nature (Lond.) 266, 745–746 (1977)
Nuti, M.P., Ledeboer, A.M., Lepidi, A.A., Schilperoort, R.A.: Large plasmids in different Rhizobium species. J. gen. Microbiol. 100, 241–248 (1977)
Olsen, R.H., Gonzalez, C.: Escherichia coli gene transfer to unrelated bacteria by a histidine operon—RP1 drug resistant plasmid complex. Biochem. Biophys. Res. Commun. 59, 377–385 (1974)
Olsen, R.H., Shipley, P.: Host range and properties of the Pseudomonas aeruginosa R factor R1822. J. Bact. 133, 772–780 (1973)
Roberts, R.J.: Restriction endonucleases. Critical Reviews in Biochemistry 4, 123–164 (1976)
Rolfe, B., Holloway, B.W.: Alteration in host specificity of bacterial deoxyribonucleic acid after an increase in growth temperature of Pseudomonas aeruginosa. J. Bact. 92, 43–48 (1966)
Sawula, R.V., Crawford, I.P.: Mapping of the tryptophan genes of Acinetobacter calcoaceticus by transformation. J. Bact. 112, 797–805 (1972)
Smith, D.L., Gomez Lus, R., Rulsio Calvo, M.C., Datta, N., Jacob, A.E., Hedges, R.W.: Third type of plasmid conferring gentamicin resistance in Pseudomonas aeruginosa. Antimicrob. Agents and Chemothr. 8, 227–230 (1975)
Sugden, B., De Troy, B., Roberts, R.J., Sambrook, H.: Agarose slab gel electrophoresis equipment. Analyt. Biochem. 68, 36–46 (1975)
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Communicated by Ch. Auerbach
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Johnston, A.W.B., Bibb, M.J. & Beringer, J.E. Tryptophan genes in Rhizobium—Their organization and their transfer to other bacterial genera. Molec. Gen. Genet. 165, 323–330 (1978). https://doi.org/10.1007/BF00332533
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DOI: https://doi.org/10.1007/BF00332533