Abstract
In tissue cultures of male Microtus agrestis, diploid mitoses with two X or two Y chromosomes were found. For identifiying the sex chromosomes in nonhypotonioally treated mitoses, the asynchrony of DNA replication of the sex chromosomes of both sexes was used. The constitutive heterochromatin of Y replicates later in the S period than X, and X2 of the female replicates later than X1. Autoradiographic studies of tetraploid tripolar mitoses showed that the diploid daughter nuclei contain either XX or YY in the male; in the female, X1X2 daughter nuclei were found less frequently than X1X1 and X2X2 cells.
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Pera, F., Rainer, B. Studies of multipolar mitoses in euploid tissue cultures. Chromosoma 46, 225–232 (1974). https://doi.org/10.1007/BF00332520
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DOI: https://doi.org/10.1007/BF00332520