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Cytogenetic studies of chromosome replication in Zea mays L.: Regulation of homologue synchrony

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Abstract

Maize stocks with or without additional heterochromatin (a B chromosome or abnormal chromosome 10), and a stock heteromorphic for a reciprocal translocation, were used in a study of homologue synchrony of replication. Batches of attached primary roots were pulse labelled with 3H-TdR and sampled at hourly intervals during the S period (at 18° C). Silver grain counts over each chromosome arm from 25 cells at each collection period were submitted to analyses. Qualitative synchrony (concordance) was tested between chromosome arms using a χ2 test. Quantitative synchrony was tested with an I-divergence test. Results from these tests in the control experiments were consistent with the hypothesis of meta-synchrony of homologue replication. The addition of heterochromatin in the form of a B chromosome or an abnormal 10 chromosome increased asynchrony two-fold. The insertion of a heteromorphic translocation into the genome produced a similar though less pronounced effect.

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Authors and Affiliations

  1. Department of Genetics, Health Sciences Center, Winnipeg, Manitoba

    George R. Douglas & David B. Walden

  2. Department of Plant Sciences, University of Western Ontario, London, Ontario

    George R. Douglas & David B. Walden

  3. Human Development Division Department of Health and Welfare, Environmental Health Center Tunney's Pasture, K1A 0L2, Ottawa, Ontario, Canada

    George R. Douglas

Authors
  1. George R. Douglas
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  2. David B. Walden
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Douglas, G.R., Walden, D.B. Cytogenetic studies of chromosome replication in Zea mays L.: Regulation of homologue synchrony. Chromosoma 46, 13–22 (1974). https://doi.org/10.1007/BF00332335

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  • DOI: https://doi.org/10.1007/BF00332335

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