Summary
Restriction fragments containing either leut (a rho-independent transcription termination site) and/or leut' (a rho-dependent transcription termination site) were cloned into plasmid pOL4. Treatment of plasmid-containing Escherichia coli strains with coumermycin resulted in loss of in vivo plasmid superhelicity 10 min after antibiotic addition. Galactokinase levels specified by these plasmid-containing strains were the same regardless of whether functional DNA gyrase was present. These results suggest that transcription termination is unaffected by the superhelical state of DNA.
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References
Adams CW, Hatfield GW (1984) Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors. J Biol Chem 259:7399–7403
Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 47:180–230
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Calvo JM, Freundlich M, Umbarger HF (1969) Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: Isolation of regulatory mutants. J Bacteriol 97:1272–1282
DiNardo S, Voelkel KA, Sternglanz R, Reynolds AE, Wright A (1982) Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell 31:43–51
Drlica K (1984) Biology of bacterial deoxyribonucleic acid topoisomerases. Microbiol Rev 48:273–289
Drlica K, Snyder M (1978) Superhelical Escherichia coli DNA: relaxation by coumermycin. J Mol Biol 120:145–154
Dubnau E, Margolin P (1972) Suppression of promoter mutations by the pleiotropic supX mutations. Mol Gen Genet 117:91–112
Friedberg D, Rosenthal ER, Jones JW, Calvo JM (1985) Characterization of the 3′ end of the leucine operon of Salmonella typhimurium. Mol Gen Genet 199:486–494
Gellert M (1981) DNA topoisomerases. Annu Rev Biochem 50:879–910
Gellert M, O'Dea MH, Itoh T, Tomizawa J-I (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci USA 73:4474–4478
Gellert M, Mizuuchi M, O'Dea M, Itoh T, Tomizawa J-I (1977) Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity. Proc Natl Acad Sci USA 74:4772–4776
Graf LM Jr, Burns RO (1973) The supX/leu-500 mutations and expression of the leucine operon. Mol Gen Genet 126:291–301
Groves W, Davis F, Sells B (1968) Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem 22:195–210
Haughn GW, Squires CM, DeFelice M, Lago CT, Calvo JM (1985) Unusual organization of the ilvIH promoter of Escherichia coli. J Bacteriol 163:186–198
Honigman A, Mahajna J, Alturia S, Koby S, Teff D, Locker-Giladi H, Hyman H, Kronman C, Oppenheim AB (1985) Plasmid vectors designed for the analysis of transcription termination signals. Gene 36:131–141
Koch AL (1970) Turbidity measurements of bacterial cultures in some available commercial instruments. Anal Biochem 38:252–259
Kubo M, Kano Y, Nakamura M, Nagata A, Imamoto F (1979) In vivo enhancement of general and specific transcription in Escherichia coli by DNA gyrase activity. Gene 7:153–171
Lederberg EM, Cohen SN (1974) Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol 119:1072–1074
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
McKenney K, Shimatake H, Court D, Schmeisner U, Brady C, Rosenberg M (1981) A system to study promoter and terminator signals recognized by E. coli RNA polymerase. In: Chirikjiam JG, Papas TS (eds) Gene amplification and analysis, vol 2. Elsevier-North-Holland, Amsterdam, pp 383–415
Menzel R, Gellert M (1983) Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling. Cell 34:105–113
Mirkin SM, Shmerling ZhG (1982) DNA replication and transcription in a temperature-sensitive mutant of E. coli with a defective DNA gyrase B subunit. Mol Gen Genet 188:91–95
Mirkin SM, Zaitsev EN, Panyutin IG, Lyamichev VI (1984) Native supercoiling of DNA: the effects of DNA gyrase and ω protein in E. coli. Mol Gen Genet 196:508–512
Morgan WD, Bear DG, Litchman BL, von Hippel PH (1985) RNA sequence and secondary structure requirements for rho-dependent transcription termination. Nucleic Acids Res 13:3739–3754
Mukai FH, Margolin P (1963) Analysis of unlinked suppressors of an 0° mutation in Salmonella. Proc Natl Acad Sci USA 50:140–148
Platt T (1981) Termination of transcription and its regulation in the tryptophan operon of E. coli. Cell 24:10–23
Pruss GJ, Drlica K (1985) DNA supercoiling and suppression of the leu-500 promoter mutation. J Bacteriol 164:947–949
Pruss GJ, Manes SH, Drlica K (1982) Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes. Cell 31:35–42
Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
Rosenthal ER, Calvo JM (1987) Transcription termination sites at the distal end of the leu operon of Salmonella typhimurium. J Mol Biol, in press
Ryan MJ (1976) Coumermycin A1: a preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization. Biochemistry 15:3769–3777
Sanzey B (1979) Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase. J Bacteriol 138:40–47
Shaw WV (1975) Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol XLIII:737–755
Smith CL, Kubo M, Imamoto F (1978) Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase. Nature 275:420–423
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
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Communicated by N.D.F. Grindley
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Rosenthal, E.R., Calvo, J.M. Effect of DNA superhelicity on transcription termination. Mol Gen Genet 207, 430–434 (1987). https://doi.org/10.1007/BF00331611
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DOI: https://doi.org/10.1007/BF00331611