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The plastome of a brown alga, Dictyota dichotoma

II. Location of structural genes coding for ribosomal RNAs, the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and for polypeptides of photosystems I and II

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Summary

The plastid DNA of Dictyota dichotoma contains an inverted repeat of about 4.7 kb as determined with cloned BamHI-SalI subfragments. The repeat separates two single copy regions of 72 and 42 kb, respectively, and contains the genes for the 16 S and 23 S rRNAs. The rbcL gene was located 27 kb from the rrnA operon in the large single copy region, whereas no Southern hybridization signals could be obtained using two rbcS probes from higher plants. The genes coding for the polypeptides of photosystems I and II appear to be highly rearranged in comparison with plastomes from higher plants. The psaA and psaB genes map closely together inside the large single copy region, less than 8 kb from the rrnA operon. About 60 kb separates these genes from a second locus hybridizing with the psaA probe. Genes for photosystem II associated polypeptides (psbA-E) are spread over both single copy regions. The close association of the psbC and psbD genes within a 4.1 kb BamHI-BglII subfragment suggests that they may form a single transcriptional unit as in higher plants.

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Abbreviations

ptDNA:

plastid DNA

B:

BamHI

Bg:

BglII

BBg:

BamHI-BglII

S:

SalI

BS:

BamHI-SalI

IR:

inverted repeat

SSC:

0.15 M sodium chloride, 0.015 M sodium citrate

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Authors and Affiliations

  1. Botanisches Institut, Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf, Federal Republic of Germany

    Maria Kuhsel & Klaus V. Kowallik

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  1. Maria Kuhsel
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  2. Klaus V. Kowallik
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Communicated by R.G. Herrmann

Dedicated to Prof. W. Nultsch on occasion of his 60th birthday

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Kuhsel, M., Kowallik, K.V. The plastome of a brown alga, Dictyota dichotoma . Mol Gen Genet 207, 361–368 (1987). https://doi.org/10.1007/BF00331602

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  • DOI: https://doi.org/10.1007/BF00331602

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