Summary
An in situ hybridization method was developed for detecting single or low copy number genes in metaphase chromosomes of plants. Using as a probe 3H-labelled plasmid pABDI, which confers kanamycin resistance (Kmr) to transformed cells. DNA introduced into the plant genome by direct gene transfer was detected with a high efficiency: about 60% to 80% of interphase and metaphase plates showed a strong signal. The insertion site of the Kmr gene in two independent transformants was localised on different homologous chromosome pairs. This result independently confirmed previous genetic data which had indicated that transformed DNA was integrated into plant chromosomes in single blocks.
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Communicated by J. Schell
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Mouras, A., Saul, M.W., Essad, S. et al. Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Mol Gen Genet 207, 204–209 (1987). https://doi.org/10.1007/BF00331579
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DOI: https://doi.org/10.1007/BF00331579