Summary
A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.
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Communicated by B.J. Kilbey
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Debenham, P.G., Webb, M.B.T. & Law, J. The cloning of the rorB gene of Escherichia coli . Mol Gen Genet 215, 156–160 (1988). https://doi.org/10.1007/BF00331318
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DOI: https://doi.org/10.1007/BF00331318