Summary
Oligomycin-resistant lines were derived from a Nicotiana sylvestris cell suspension, after N-nitroso-N-methylurea mutagenesis followed by selection in the presence of 0.4 μg/ml oligomycin, a specific mitochondrial ATPase inhibitor. One of the lines, oli R38 was further analyzed to investigate the role of mitochondria in this resistance. The oli R38 line proved to be also highly resistant to venturicidin, another specific inhibitor of mitochondrial ATPase. By the donor-recipient protoplast-fusion procedure the cytoplasmic organelles of oli R38 were transferred to protoplasts of Line 92, a line of tobacco plants which contain the cytoplasmic organelles of N. undulata. Cell suspensions prepared from several cybrid plants, containing the cytoplasmic organelles of oli R38, exhibited the same level of oligomycin resistance as the oli R38 line.
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Aviv, D., Galun, E. Transfer of cytoplasmic organelles from an oligomycin-resistant Nicotiana cell suspension into tobacco protoplasts yielding oligomycin-resistant cybrid plants. Mol Gen Genet 215, 128–133 (1988). https://doi.org/10.1007/BF00331313
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DOI: https://doi.org/10.1007/BF00331313