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The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae

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Summary

We have cloned and sequenced the PGI1 gene, encoding phosphoglucose isomerase (E.C.5.3.1.9), from Saccharomyces cerevisiae. The nucleotide sequence predicts subunits of 554 amino acids with a molecular weight of 61230. Both the size and amino acid composition correlate well with measurements from purified protein. We have compared the PGI1 protein with the predicted sequence for pig muscle PGI. In spite of some evolutionary divergence the proteins are very similar and there are some highly conserved regions, two of which have been implicated in the active site. It has been suggested that PGI exists in two or more isozyme forms in S. cerevisiae and analogy with ADR2/ADC1 suggests that such PGI isozymes might also be differentially regulated during glycolytic/gluconeogenic growth. We have used accurate quantitation of PGI1 mRNA and gene fusions of PGI1 to the lacZ gene of Escherichia coli to show that PGI1 transcription is regulated neither between glycolytic and gluconeogenic growth nor between exponential and stationary phase. The complete lack of PGI activity in PGI1 deletion mutants and of differential regulation suggests that the isozymes of PGI might result merely from processing of the PGI1 gene product.

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Author notes

  1. Jeremy B. A. Green

    Present address: Institut für Mikrobiologie, Technische Hochschule Darmstadt, Schnittspahnstrasse 10, D-6100, Darmstadt, Germany

  2. Anthony P. H. Wright

    Present address: Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, S-141 86, Huddinge, Sweden

  3. Wing Y. Cheung

    Present address: CSIRO Division of Horticultural Research, GPO Box 300, 5001, Adelaide, SA, Australia

  4. William E. Lancashire

    Present address: Whitbread R&D Genetics Section, Leicester Biocentre, University Road, LE1 7RH, Leicester, UK

Authors and Affiliations

  1. Centre for Biotechnology, Imperial College, SW7 2AZ, London, UK

    Jeremy B. A. Green, Anthony P. H. Wright, Wing Y. Cheung, William E. Lancashire & Brian S. Hartley

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  1. Jeremy B. A. Green
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  2. Anthony P. H. Wright
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  4. William E. Lancashire
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Communicated by C.P. Hollenberg

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Green, J.B.A., Wright, A.P.H., Cheung, W.Y. et al. The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae . Mol Gen Genet 215, 100–106 (1988). https://doi.org/10.1007/BF00331310

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  • DOI: https://doi.org/10.1007/BF00331310

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