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Plants regenerated from auxin-auxotrophic variants are inviable

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Summary

Shoots regenerated from auxin-auxotrophic variants of Nicotiana plumbaginifolia were inviable when cultured in vitro in the absence of auxin. Variant shoots survived longer when grafted to wild-type stocks but eventually died after a characteristic pattern of degeneration. The auxin auxotrophs were isolated after mutagen treatment by a total isolation method as infrequent variants amongst haploid protoplast-derived cell colonies. The variants responded to several active auxins but, unlike the wild type, not to cytokinin. Plant regeneration from variant cultures ceased at early stages of shoot formation after complete withdrawal of auxin from the medium.

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Abbreviations

BAP:

6-benzylaminopurine

BUdR:

5-bromo-2′-deoxyuridine

2,4-D:

2,4-dichlorophenoxyacetic acid

DMSO:

dimethyl sulfoxide

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MNNG:

N-methyl-N′-nitro-N-nitrosoguanidine

NAA:

1-naphthaleneacetic acid

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Author notes

  1. Tiina Vahala

    Present address: Institute of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppasala, Sweden

  2. Maarten Koornneef

    Present address: Department of Genetic, Agricultural University, 53 Generaal Foulkesweg, NL-6703 BM, Wageningen, The Netherlands

Authors and Affiliations

  1. Friedrich Miescher-Institut, Postfach 2543, CH-4002, Basel, Switzerland

    Anne D. Blonstein, Tiina Vahala, Maarten Koornneef & Patrick J. King

Authors
  1. Anne D. Blonstein
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  2. Tiina Vahala
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  3. Maarten Koornneef
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  4. Patrick J. King
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Communicated by J. Schell

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Blonstein, A.D., Vahala, T., Koornneef, M. et al. Plants regenerated from auxin-auxotrophic variants are inviable. Mol Gen Genet 215, 58–64 (1988). https://doi.org/10.1007/BF00331303

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  • DOI: https://doi.org/10.1007/BF00331303

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