Summary
Nonsense codon suppressing lysogens of E. coli have been made using ϕ80 psu +3 -A2 and ϕ80 psu +oc -A2, heat-sensitive amber and ochre suppressing derivatives, respectively, of bacteriophage ϕ80. The various lysogens selected differ in strength of suppression as well as in heat sensitivity of suppressor function. Heat-resistant derivatives, some still carrying the A2 mutation, can be selected from the heat sensitive parents. Mapping expreiments indicate that the ϕ80 derivatives integrate at the tyrTV locus, which contains two copies of tRNA Tyr1 . The origin of the various suppressor phenotypes appears to be related to the great variety of distinctive recombination events possible either between the incoming tRNA Tyr1 gene and the host copies, or among the three copies of this gene in the lysogens.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altman S (1976) A modified uridine in the anticodon of E. coli tRNA Tyr1 su +oc . Nucl Acids Res 3:441–448
Altman S, Brenner S, Smith JD (1971) Identification of an ochresuppressing anticodon. J Mol Biol 56:195–197
Altman S, Smith JD (1971) Tyrosine tRNA precursor molecule polynucleotide sequence. Nature New Biol 233:35–39
Brenner S, Beckwith JR (1965) Ochre mutants, a new class of suppressible nonsense mutants. J Mol Biol 13:629–637
Celis JE, Hooper ML, Smith JD (1973) Amino acid acceptor stem of E. coli suppressor tRNATyr is a site of synthetase recognition. Nature New Biol 244:261–264
Feinstein SI, Altman S (1977) Coding properties of an ochre-suppressing derivative of Escherichia coli tRNA Tyr1 . J Mol Biol 112:453–470
Feinstein SI, Altman S (1978) Context effects on nonsense codon suppression in Escherichia coli. Genetics 88:201–219
Fiandt M, Hradecna Z, Lozeron HA, Szybalski W (1971) Electron micrographic mapping of deletions, insertions, inversions and homologies in the DNAs of coliphages lambda and phi-80. In: Hershey AD (ed) The bacteriophage Lambda, pp 329–354. New York, Cold Spring Harbor Laboratory
Ghysen A, Celis JE (1974) Joint transcription of two tRNA Tyr1 genes from Escherichia coli. Nature 249:418–421
Hooper ML (1971) Studies on amber suppressor tyrosine transfer RNA. D Phil Thesis. Cambridge University, Cambridge, England
Hooper ML, Russell RL, Smith JD (1972) Mischarging in mutant tyrosine transfer RNAs. FEBS Lett 22:149–155
Low KB, Gates F, Goldstein T, Soll D (1971) Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetase. J Bacteriol 108:742–750
Luria SE, Burrous JW (1957) Hybridization between Escherichia coli and Shigella. J Bacteriol 74:461–476
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY
Norkin LC (1970) Marker specific effects in genetic recombination. J Mol Biol 51:633–656
Rossi JJ, Landy A (1979) Structure and organization of the two tRNATyr gene clusters on the E. coli chromosome. Cell 16:523–534
Russell RL, Abelson JN, Landy A, Gefter ML, Brenner S, Smith JD (1970) Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol 47:1–13
Smith JD, Barnett L, Brenner S, Russell RL (1970) More mutant tyrosine transfer ribonucleic acids. J Mol Biol 54:1–14
Yahata H, Ocada Y, Tsugita A (1970) Adjacent effect on suppression efficiency. II. Study on ochre and amber mutants of T4 phage lysozyme. Mol Gen Genet 106:208–212
Zipser D (1967a) Orientation of nonsense codons on the genetic map of the lac operon. Science 157:1176–1177
Zipser D (1967b) UGA: A third class of suppressible polar mutants. J Mol Biol 29:441–445
Author information
Authors and Affiliations
Additional information
Communicated by L. Lerman
Rights and permissions
About this article
Cite this article
Feinstein, S.I., Altman, S. Unusual suppression properties of lysogens containing derivatives of ϕ80 psu +3 . Molec Gen Genet 187, 347–353 (1982). https://doi.org/10.1007/BF00331142
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331142