Summary
The early effects of thyroxine on RNA-metabolism in various tissues of Xenopus-larvae have been investigated by autoradiography using uridine-3H as precursor. In the presence of thyroxine (T4) a stimulation of nuclear RNA-synthesis is detected already 4 h after the onset of hormone administration. There are tissue-specific differences in the extent and the time course of response. T4 most effectively stimulates RNA-synthesis after 24 h of pretreatment. During 72 h of hormone treatment there is no detectable shift of labeled nuclear RNA into the cytoplasm.
Actinomycin D inhibits T4-induced RNA-synthesis already after 4 h, but is most effective after 24 h of pretreatment. Puromycin inhibits RNA-synthesis to a minor extent, but only if exposure to the antibiotic precedes hormone administration.
In view of the extent of inhibition of RNA-synthesis by actinomycin D, it is concluded that T4-induced metamorphic responses not only involves transcription of specific mRNA's, but must also depend upon formation of other types of RNA.
Zusammenfassung
Die Frühwirkung von Thyroxin auf den RNS-Stoffwechsel in verschiedenen Geweben von Xenopus-Larven wurde autoradiographisch mit Uridin-3H untersucht. Bereits nach 4 h wird in Anwesenheit von Thyroxin (T4) eine Stimulation der nukleären RNS-Synthese festgestellt. Dabei sind das Ausmaß und der zeitliche Verlauf je nach Gewebe verschieden. Die RNS-Synthese wird nach einer T4-Vorbehandlung von 24 h am stärksten stimuliert. Während 72 h Hormon-Behandlung kann kein Transport markierter RNS vom Zellkern ins Cytoplasma festgestellt werden.
Aetinomycin D hemmt die T4-induzierte RNS-Synthese bereits nach 4 h. Die stärkste Hemmung tritt nach 24stündiger Vorbehandlung auf. Puromycin hemmt die RNS-Synthese nur, wenn das Antibiotikum 24 h vor dem Hormon appliziert wird.
Angesichts der starken Hemmung der RNS-Synthese durch Aetinomycin D wird angenommen, daß die T4-induzierten Veränderungen während der Metamorphose nicht nur durch Transkription spezifischer mRNS-Arten bedingt sind, sondern auch von der Bildung anderer RNS-Typen abhängen.
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Stadler, H. Autoradiographische Untersuchungen über die Frühwirkung von Thyroxin auf die RNS-Synthese in verschiedenen Geweben von Xenopus-Larven. Z. Zellforsch. 117, 118–138 (1971). https://doi.org/10.1007/BF00331106
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DOI: https://doi.org/10.1007/BF00331106