Abstract
We have analysed trisomic pea plants by DNA/ DNA hybridization techniques, exploiting the copy number excess of DNA sequences in trisomics, together with a knowledge of their genetics, in order to address problems in the correlation of genetic and cytogenetic information. One such long-standing problem in pea genetics is the assignment of one rDNA locus (Rrn2) to a linkage group on chromosome 7. Our results show that Rrn2 does not reside on the same chromosome as Lg-1 or Vc-2 which we have previously shown to be linked to r, a linkage group 7 specific marker.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beckmann JS, Soller M (1986) Restriction fragment length polymorphisms in plant genetic improvement. Oxford Surv Plant Mol Cell Biol 3:196–250
Blixt S (1974) The pea. In: King RC (ed) Handbook of Genetics, vol 2. Plenum Press, New York, pp 181–221
Casey R, Domoney C, Ellis THN (1986) Legume storage proteins and their genes. Oxford Surv Plant Mol Cell Biol 3:1–95
Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646
Domoney C, Casey R (1984) Storage protein precursor polypeptides in cotyledons of Pisum sativum L. Identification of, and isolation of a cDNA clone for, an 80,000-Mr legumin-related polypeptide. Eur J Biochem 139:321–327
Domoney C, Casey R (1985) Measurement of gene number for seed storage protein in Pisum. Nucleic Acids Res 13:687–699
Domoney C, Barker D, Casey R (1986a) The complete deduced amino acid sequences of legumin β-polypeptides from different genetic loci in Pisum. Plant Mol Biol 7:467–474
Domoney C, Ellis THN, Davies DR (1986b) Organization and mapping of legumin genes in Pisum. Mol Gen Genet 202:280–285
Ellis THN, Davies DR, Castleton JA, Bedford ID (1984) The organization and genetics of rDNA length variants in peas. Chromosoma 91:74–81
Ellis THN, Domoney C, Castleton J, Cleary W, Davies DR (1986) Vicilin genes of Pisum. Mol Gen Genet 205:164–169
Folkeson D (1984) Free segregation between a (3S–7S) interchange and genes within linkage group VII in Pisum sativum. Hereditas 101:227–233
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
Lamm R (1951) Cytological studies in Pisum. Hereditas 37:356–372
Lamm R (1981) Giemsa C-banding and silver staining for cytological studies in Pisum. Hereditas 94:45–52
Polans NO, Weeden NF, Thompson WF (1986) Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea. Theor Appl Genet 72:289–295
Rusche JR, Howard-Flanders P (1985) Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase. Nucleic Acids Res 13:1997–2008
Vieira J, Messing J (1982) The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Weeden N (1985) An isozyme linkage map for Pisum sativum. In: Hebblethwaite P, Dawkins T, Heath M (eds) The pea crop — a basis for improvement. Butterworths, Guildford, pp 55–66
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ellis, T.H.N., Cleary, W. Trisomy: a useful adjunct to RFLP mapping in pea. Chromosoma 96, 91–94 (1988). https://doi.org/10.1007/BF00331039
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331039