Summary
The properties of four radiation sensitive ts mutants of the yeast Saccharomyces paradoxus, txs 1, txs 2, txs 4and txs 5,were studied. Genetic analysis proves the mutants to be non-allelic. The mutants are sensitive to X-rays and ultraviolet. Holding at 37°C in a complete medium but not in water sharply decreases the viability. With txs 2this effect could also be observed in water. The mutants formed filaments and showed abnormal cytokinesis under restrictive conditions, this effect being the most pronounced with txs 4.The frequency of mitotic recombiantion induced by X-rays and UV is much lower in the mutants (except txs 2)as compared to the wild type. Restrictive temperature somewhat increased the mitotic recombination in the mutants (excepts txs 1).Shift of the mutants into restrictive temperature leads to immediate, inhibition of DNA synthesis; the extent of this inhibition correlates with the levels of radiation and temperature sensitivity, both being higher in txs 2and txs 4.Elevated temperature also decreased RNA and protein synthesis in txs 2.It is suggested that mutation txs 2affects a function participating at the last stages in postirradiation repair of DNA, probably DNA ligase, txs 4seemed to affect the process of nuclear division.
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Bandas, E.L., Bekker, M.L., Luchkina, L.A. et al. Temperature sensitive radiosensitive mutants of the yeast Saccharomyces paradoxus . Molec. gen. Genet. 126, 153–164 (1973). https://doi.org/10.1007/BF00330990
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DOI: https://doi.org/10.1007/BF00330990