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Genetic analysis of essential genes in the ftsE region of the Escherichia coli genetic map and identification of a new cell division gene, ftsS

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Summary

Several conditional lethal mutants of Escherichia coli have been analysed genetically using generalized transduction and lambda transducing vectors. Three temperature-sensitive ftsE mutants were found as was a cold-sensitive ftsE mutant. A new gene was foud which mapped close to ftsE, namely ftsS. Both cell division genes map close to the gene which controls the heat-shock regulon (htpR).

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References

  • Anderson JJ, Oxender DL (1977) Escherichia coli transport mutants lacking binding protein and other components of the branched-chain amino acid transport systems. J Bacteriol 130:384–392

    Google Scholar 

  • Bachmann B (1983) Linkage map of Escherichia coli K12, edition 7. Microbiol Rev 47:180–230

    Google Scholar 

  • Bachmann B, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Begg KJ, Hatfull GF, Donachie WD (1980) Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ. J Bacteriol 44:435–437

    Google Scholar 

  • Borck K, Beggs JD, Brammmar WJ, Hopkins AS, Murray NE (1976) The construction in vitro of transducing derivatives of phage Lambda. Mol Gen Genet 146:199–207

    Google Scholar 

  • Donachie WD, Begg, KJ, Vicente M (1976) Cell length, cell growth and cell division. Nature (London) 264:328–333

    Google Scholar 

  • Glassberg J, Meyer RR, Kornberg A (1979) Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol 140:14–19

    Google Scholar 

  • Helmstetter CE, Pierucci O, Weinberger M, Holmes M, Tang MS (1979) Control of cell division in Escherichia coli. In: Sokatch JR, Ornsten LN (eds) The bacteria, vol VII. Academic Press, London and New York, pp 517–579

    Google Scholar 

  • Kleckner N, Barker DF, Ross DG, Botstein D (1978) Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics 90:427–461

    Google Scholar 

  • Lutkenhaus JF, Donachie WD (1979) Identification of the ftsA gene product. J Bacteriol 137:1088–1094

    Google Scholar 

  • Lutkenhaus JF, Wu HC (1980) Determination of transcrptional units and gene products from the ftsA region of Escherichia coli. J Bacteriol 143:1281–1288

    Google Scholar 

  • Lutkenhaus JF, Wolf-Watz H, Donachie WD (1980) Organization of genes in the ftsA-envA region of the Escherichia coli map and identification of a new fts locus (ftsZ). J Bacteriol 142:615–620

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York

    Google Scholar 

  • Neidhardt FC, Van Bogelen RA, Lau ET (1983) Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli. J Bacteriol 153:597–603

    Google Scholar 

  • Ricard M, Hirota Y (1973) Process of cellular division in Escherichia coli: Physiological study on thermosensitive mutants defective in cell division. J Bacteriol 116:314–322

    Google Scholar 

  • Salmond GPC, Lutkenhaus JF, Donachie WD (1980) Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG. J Bacteriol 144:438–440

    Google Scholar 

  • Sevastopoulos CG, Wehr CT, Glaser DA (1971) Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication. Proc Natl Acad Sci USA 74:3485–3489

    Google Scholar 

  • Sturgeon JA, Ingram LO (1978) Low temperature conditional division mutants of Escherichia coli. J Bacteriol 133:256–264

    Google Scholar 

  • Torti S, Park JT (1980) Genetic characterization of a filamentforming lipoprotein-deficient mutant of Escherichia coli. J Bacteriol 143:1289–1294

    Google Scholar 

  • Wilson GG, Young KKY, Edlin GJ, Konigsberg W (1979) High frequency generelized transduction by bacteriophage T4. Nature (London) 280:80–81

    Google Scholar 

  • Yamamori T, Yura T (1982) Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K12. Proc Natl Acad Sci USA 79:860–864

    Google Scholar 

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Author notes

  1. George P. C. Salmond

    Present address: Department of Biological Sciences, University of Warwick, CV4 7AL, Coventry, U.K.

  2. Stella Plakidou

    Present address: Biotechnology Centre, Cranfield Institute of Technology, Bedford, U.K.

Authors and Affiliations

  1. Biological Laboratory, The University, Canterbury, Kent, U.K.

    George P. C. Salmond & Stella Plakidou

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  1. George P. C. Salmond
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  2. Stella Plakidou
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Communicated by C. Auerbach

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Salmond, G.P.C., Plakidou, S. Genetic analysis of essential genes in the ftsE region of the Escherichia coli genetic map and identification of a new cell division gene, ftsS . Mol Gen Genet 197, 304–308 (1984). https://doi.org/10.1007/BF00330978

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  • DOI: https://doi.org/10.1007/BF00330978

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